连续流动法测定烟草中的蛋白质含量  

Determination of Protein Content in Tobacco with Continuous Flow Analytical Method

作　　者：孔浩辉[1] 郭文[1] 张心颖[1] 马青[1] 梁曼娜[1] 

机构地区：[1]广东中烟工业有限责任公司技术中心

出　　处：《烟草科技》2009年第11期44-46,共3页Tobacco Science & Technology

摘　　要：为了快速测定烟草中的蛋白质氮含量,利用烟草可溶性蛋白在0.5%热醋酸中发生沉淀的性质,先用0.5%热醋酸萃取出烟草可溶性氮中的非蛋白质氮,再用连续流动法测定,并由烟草总氮量减去非蛋白质氮量计算烟草的蛋白质氮含量。结果表明:该方法的变异系数(CV)小于3%;在90%的置信区间内,其测定值与现有的标准方法没有显著差异:t=1.082<t0.1,15=1.753。In order to determine the protein nitrogen in tobacco fast, the non-protein nitrogen in soluble nitrogen was extracted from tobacco sample with heated 0.5% acetic acid, in which soluble proteins in tobacco precipitated, and then determined with a continuous flow analyser. The protein nitrogen content in tobacco was figured out by subtracting the determined non-protein nitrogen from total nitrogen. The results showed that the CV of the method was less than 3%. There was no significant difference between the values determined by this method and current industry standard method at the confidence interval of 90%.

关 键 词：连续流动分析法 烟草 非蛋白质氮 蛋白质 

分 类 号：TS411.1[农业科学—烟草工业]