非套管法大鼠异体原位单肺移植动物模型的建立  

The experimental model of orthotopic left allograft lung transplantation in rats without using cuff

作　　者：陈刚[1] 苗锋[1] 马勤运[1] 陈佶[1] 施梦[1] 朱勇俊[1] 陈志明[1] 

机构地区：[1]复旦大学附属华山医院胸外科,上海200040

出　　处：《复旦学报（医学版）》2009年第6期727-730,共4页Fudan University Journal of Medical Sciences

摘　　要：目的建立稳定的大鼠非套管法异体原位左肺移植模型。方法使用250～350 g Sprague Dawley（SD）大鼠进行实验,采用显微外科技术,分别连续缝合支气管和肺动、静脉,建立大鼠异体原位左肺移植模型。观察大鼠存活时间,移植肺脏血管、支气管吻合口通畅性。结果10只接受左肺移植的受体大鼠均成功脱离呼吸机,供肺冷缺血时间约40 min,热缺血时间约40 min,总手术时间约130 min。解剖后检查示各吻合口均通畅。结论非套管法大鼠原位左肺移植模型具有稳定、可靠、接近临床肺移植的优点。Objective To establish a stable model of rat orthotopic left lung transplantation using direct suture of vessels and bronchi. Methods Ten Sprague Dawley （SD） rats weighted 250 to 350 g were used as lung donors and recipients respectively. Airway and pulmonary vessels were reconstructed microsurgically using continuous running suture technique. Survival time were recorded and donor lungs were checked by autopsy. Results All 10 rats received left lung transplantation were weaned from ventilator successfully. Both of cold ischemia time and warm ischemia time were about 40 minutes. The total procedure took about 130 minutes. Autopsy was used to check the patency of anastomotic sites. No thrombosis or air leak was found. Conclusions Direct microsurgical surture can be used to establish an experimental model of orthotopic left allograft lung transplantation in rats. This method is proved to be stable, reliable and similar to clinical practices.

关 键 词：肺移植 原位移植 实验动物模型 SD大鼠 

分 类 号：R655.3[医药卫生—外科学]