EPO对缺血/再灌注损伤大鼠心肌细胞Mfn2蛋白表达及心肌细胞凋亡的影响  

EFFECT OF RECOMBINANT HUMAN ERYTHROPOIETIN (rh-EPO) ON Mfn2 PROTEIN EXPRESSION AND MYOCARDIAL APOPTOSIS IN RATS WITH ISCHEMIA-REPERFUSION INJURY

作　　者：杨世明[1] 孙瑜[2] 涂志业[3] 糜涛[4] 陈莉莉[4] 郭小梅[3] 

机构地区：[1]华中科技大学同济医学院解剖学系组织胚胎学教研室 [2]华中科技大学同济医学院附属同济医院心胸外科,武汉430030 [3]华中科技大学同济医学院附属同济医院心血管内科,武汉430030 [4]华中科技大学同济医学院附属同济医院综合内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2009年第6期623-627,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(3060023330672206)

摘　　要：目的观察重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对缺血/再灌注损伤大鼠心肌细胞Mitofusin2(Mfn2)蛋白表达的影响及其抗心肌细胞凋亡的作用。方法选取成年SD大鼠35只,随机分为正常组(Normal),假手术组(Sham),缺血再灌注组(I/R),缺血再灌注EPO治疗组(I/R+EPO)。各组分别于再灌注3h和24h后,剪取心脏缺血/再灌注损伤区域,用脱氧核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测心肌细胞凋亡,免疫组化法检测Mfn2蛋白的表达。结果再灌注3h和24h后,与正常组和假手术组相比,I/R组Mfn2蛋白的表达和心肌细胞凋亡均显著增加;与I/R组相比,I/R+EPO组Mfn2蛋白的表达和心肌细胞凋亡均显著降低。结论EPO可以下调缺血再灌注损伤后心肌细胞Mfn2蛋白的表达,抑制心肌细胞的凋亡。Objective To investigate the effect of recombinant human erythropoietin（rh-EPO） on Mfn2 protein expression and myocardial apoptosis in rats with ischemia-reperfusion（I/R） injury.Methods35 adult SD rats were divided randomly into 4 groups： normal group,sham group,ischemia-reperfusion group（I/R）,and EPO treatment group（I/R＋EPO）.3 hours and 24 hours after reperfusion,the I/R injury areas of rat hearts were harvested,and myocardial apoptosis was assessed by in situ terminal deoxynucleotidyl transferase（TdT）-dUTP nick-end labeling（TUNEL staining） and Mfn2 protein expression was detected by immunohistochemistry among all groups.Results3 hours and 24 hours after reperfusion,compared with those of the normal and sham group,myocardial apoptosis and Mfn2 p0rotein expression of the I/R group remarkably increased;compared with those of the I/R group,myocardial apoptosis and Mfn2 protein expression of I/R＋EPO group significantly decreased.Conclusion EPO can down regulate myocardial Mfn2 protein expression and decrease myocardium apoptosis after ischemia-reperfusion injury in rats.

关 键 词：促红细胞生成素 缺血/再灌注损伤 心肌细胞 凋亡 Mitofusin2(Mfn2) 

分 类 号：R542.22[医药卫生—心血管疾病]