异氟醚对老龄大鼠海马CA3区突触素表达的影响  

Effect of isoflurane on synaptophysin expression in hippocampal CA3 area in aged rats

作　　者：李丹[1] 欧阳文[1] 段开明[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,长沙市410013

出　　处：《中华麻醉学杂志》2009年第10期899-902,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30871306）

摘　　要：目的 评价异氟醚对老龄大鼠海马CA3区突触素表达的影响。方法雌性清洁级SD大鼠63只，月龄24月，体重400～650g，随机分为3组（n=21）：对照组（C组）、1．2％异氟醚组（E1组）和1．8％异氟醚组（E2组）C组吸入含40％氧气的空氧混合气体3h；E1,2组吸入3％异氟醚行麻醉诱导，待翻正反射消失后再分别吸入1．2％、1．8％异氟醚维持3h。各组随机取12只大鼠，于麻醉结束后第1天采用Morris水迷宫系统测定大鼠认知功能（逃避潜伏期和探索时间），连续测定7d；随机取9只大鼠于麻醉结束后第1、3和7天处死，测定海马CA3区突触素的表达水平。结果与C组比较，E1组和E2组麻醉结束后第2、3天逃避潜伏期延长（P〈0．05或0．01），第4～6天逃避潜伏期差异无统计学意义（P〉0．05），麻醉结束后海马CA3区突触素表达持续下调（P〈0．05）。三组探索时间比较差异无统计学意义（P〉005）；与E1组比较，E2组逃避潜伏期差异无统计学意义（P〉0．05），海马CA3区突触素表达下调（P〈0．01）。结论异氟醚致老龄大鼠认知功能障碍与海马突触素表达无关。Objective To investigate the effect of isoflurane on the synaptophysin expression in hippocampal CA3 area in aged rats. Methods Sixty-three 24-month-old SD rats, weighing 400-650 g, were randomly divided into 3 groups （ n = 21 each） ： control group （group C） , 1.2% isoflurane group （group E1）, and 1.8% isoflurane group （group E2）, The rats in group C only inhaled a gas mixture containing 40% oxygen for 3 h. The rats in group E1 and E2, inhaled 3% isoflurane for induction, and after righting reflex disappeared, the concentration was reduced to 1.2% and 1.8% respectively which was maintained for 3 h. Twelve rats randomly selected from each group underwent Morris water maze test for assessment of learning and memory function （escape latency and probe time）. The training test was performed 4 times a day for 7 d. Nine rats randomly selected front each group were decapitated at day 1, 3, and 7 after the termination of anesthesia. The hippocampal CA3 area was isolated for detenuination of synaptophysin expression. Results The escape latency was significantly longer at day 2 and 3 after the termination of anesthesia in group E1 and E2 than in group C （ P 〈 0.05 or 0.01 ）, anti no significant difference in escape latency was found at day 4-6 after the termination of anesthesia between group E1 and E2 and group C （ P 〉 0.05）. The synaptophysin expression in hippocampal CA3 area was significantly lower in grnap E1 and E2 than in group C （P 〈 0.05）, and no significant difference in the probe time was found between the 3 grnups （P 〉 0.05）. There was no significant difference in escape latency between group E2 and group E1 （P 〉 0.05）, and the synaptophysin expression in hippocampal CA3 area was significantly lower in group E2 than in group E, （ P 〈 0.01 ） . Conclusion The cognitive dysfunction induced by isoflurane anesthesia in aged rats is not related to the synaptophysin expression in hippoeampal CA3 area.

关 键 词：异氟醚 突触素 认知障碍 老年人 

分 类 号：R96[医药卫生—药理学]