降解高浓度含酚废水菌株的筛选及性能的研究  

THE SCREENING OF STRAINS TO DEGRADE HIGH CONCENTRATION OF PHENOL-CONTAINING WASTEWATER AND STUDIES ON ITS CHARACTERISTIC

作　　者：何宗健[1] 刘敏[1] 沈越[1] 黎俊[1] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031

出　　处：《水处理技术》2009年第12期84-87,共4页Technology of Water Treatment

摘　　要：研究了含酚废水的生物降解处理过程,以培养高效菌株为主要研究对象,以石化炼油污水处理池中污水为菌种的来源,在30℃、转速为120r.min-1的恒温振荡摇床上进行菌种的驯化、培养。根据培养基中菌落的大小、形状、表面光泽、隆起程度、透明程度、边缘形状菌落颜色的差别,最终选出3种菌落分别命名为LJF-1、LJF-2、LJF-3,对3种菌株进行不同浓度含酚废水降解对比性试验。结果表明,3种株菌对不同浓度的含酚废水均有降解能力,降解能力LJF-1>LJF-3>LJF-2,从而找到了1株优势菌株LJF-1。对LJF-1进行特性研究和初步的鉴定,分别从不同温度、不同pH、不同外加碳源进行试验,发现最佳处理效果为温度30℃时,LJF-1在15h的降酚率为98.77%;pH为7.5时,在12h的降酚率是92.55%;外加碳源为1000mg·L-1时,在12h降酚率为79.8%。为LJF-1菌株应用于实际废水处理时提供参考数据。The researches processing ofbiodegradation ofphenolic wastewater treatment, takes cultivation ofhigh efficient bacteria as the research object. Wastewater polluted by phenol in coal carbonization is resource of experiment. At 30 degree of temperature, number of revolution 120r·min^-1 constant temperature concussion cradle, we take experiment toward domestication, cultivation, according to size, shape, surface color, degree of build up, degree of transient, brim shape, difference of colony of incubation media. At last, we choose three groups of colonies. They are named LJF-1,LJF-2, LJF-3 respectively. We take three experiments toward three colonies with different density phenol wastewater, and test their rate of phenol degrading in the same time period.The results show： These three bacteria have stronger impact load capability. The strongest capacity of degrading phenol is LJF-1, next to LJF-3,LJF-2.We take LJF-1 bacteria as advantageous bacteria. In order to study the advantage strains characteristics, we took experiments respectively from the different temperatures, different pH values and different concentrations of external carbon source, found conditions in the best treatment effect： when 30 degree of temperature, the rate of phenol degrading is 98.77% , in 15 h; when pH value is 7.5, the rate of phenol degrading is 92.55%, in 12 h; when the concentrations of external carbon source is 1 000 mg·L^-1, the rate of phenol degrading is 79.8%. For that the LJF-1 strains used in the actual wastewater treatment were to provide reference data.

关 键 词：含酚废水 高效菌株 降酚菌 

分 类 号：X703.1[环境科学与工程—环境工程]