无试剂型葡萄糖生物传感器的研制  

Development of A Reagentless Amperometric Glucose Biosensor

作　　者：李军华[1] 郑龙珍[1] 孙娟[1] 李欣佳[1] 

机构地区：[1]华东交通大学基础科学学院,南昌330013

出　　处：《湖北农业科学》2009年第11期2857-2860,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金(20505009);江西省自然科学基金(0620010);江西省研究生创新基金(YC08A072)

摘　　要：利用制备的羧基-β-环糊精衍生物包络二茂铁得到了羧基-β-环糊精二茂铁包络物作为新型媒介体。采用廉价易得的蛋清代替价格较高的牛血清白蛋白,用蛋清戊二醛交联媒介体、酶,成功制得了电流型葡萄糖传感器。由于羧基-β-环糊精提供的疏水空腔将二茂铁包络,减少了二茂铁的流失,另外亲水性的外壁为酶提供的良好的外环境保证了酶的活性,故该传感器具有良好的稳定性,对葡萄糖表现出良好的响应,重现性也较好,且响应时间短。检测线性范围为:1×10-5～2.68×10-3 mol.L-1,相关系数为0.9994,检测限为1.2×10-6 mol.L-1(S/N=3)。A cyclodextrin derivative（carboxyl-β-CD） was prepared and ferrocene was included into the cavity of carboxyl-β-cyclodextrin to form Fe-carboxyl-β-cyclodextrin inclusion complex（Fe-carboxyl-β-CD） as a novel mediator.A glucose biosensor which using glutaric dialdehyde and egg white which be more faciler and cheaper than BSA cross-linked with glu-cose oxidase and Fe-carboxyl-β-CD was successfully constructed.Because of ferrocene was included into the hydrophobic cavity of carboxyl-β-cyclodextrin,it was not easily washed out. Due to the hydrophile ektexine of carboxyl-β-cyclodextrin supply an excellent environment to the enzyme, the biosensor had a good response property and its stability and life time were both improved obviously. Under the optimal conditions the response of the biosensor to glucose was linear in the concentration rang of 1 ×10^-5-2.68×10^-3 mol/L, and the detection limit was 1.2×10^-6 mol/L （S/N=3）.

关 键 词：媒介体 葡萄糖氧化酶 生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]