结核病鼠T细胞γ-干扰素及白介素-2表达  

Expression level of T lymphocyte subtype INF-γ and IL-2 in tuberculosis mice

作　　者：邓国防[1] 雷建平[1] 孙丽珍[1] 

机构地区：[1]江西省胸科医院结核科,南昌330006

出　　处：《中国公共卫生》2009年第12期1458-1460,共3页Chinese Journal of Public Health

摘　　要：目的探讨结核病鼠T细胞亚群中CD4+、CD8+及CD4+CD8+(DP)T细胞γ-干扰素(IFN-γ)和白介素-2(IL-2)的表达水平。方法将30只实验小鼠随机分成对照组和结核病组,饲养6周后取血,以流式细胞仪检测分析CD4+、CD8+及(DP)T细胞内表达IFN-γ、IL-2的阳性细胞计数和各自占淋巴细胞百分率。结果表达IFN-γ的CD4+T细胞占同亚群细胞总数的比值(t=1.83±0.59)高于对照组(t=1.04±0.22),差异有统计学意义(P<0.05);表达IL-2的CD4+T细胞、CD4+CD8+(DP)T细胞占同亚群细胞总数的比值(t=2.13±0.93,t=0.06±0.04)低于对照组(t=2.86±1.78,t=0.26±0.79),差异有统计学意义(P<0.05)。结论结核病鼠CD4+T细胞表达IFN-γ的水平升高;结核病鼠CD4+、DP T细胞表达IL-2的水平降低;结核病进展期早期T细胞亚群表达IFN-γ与IL-2呈矛盾现象,提示该阶段虽然保护性免疫已经启动,但仍为非特异性免疫占优势。DENG Guo-fang,LEI Jian-ping,SUN Li-zhen.Branch of Tuberculosis,Jiangxi Provincial Chest Disease Hospital（Nanchang 330006,China） Objective To determine the expression level of T lymphocyte subtype：CD4^＋,CD8^＋ and CD4^＋CD8^＋（double positive）T cytes in tuberculosis mice.Methods Thirty mice were divided randomly into control and tuberculosis infection group according to their weight.The blood samples were taken from the mice after six weeks＇ treatment.The ratios of CD4^＋,CD8^＋ and CD4^＋CD8^＋ T lymphocyte with IFN-γ and IL-2 expression were detected with FACS Caliur flow cytometer.Results The ratio of CD4^＋ lymphocyte subtype with IFNγ expression in total T lymphocyte subtype of tuberculosis mice was higher than that of the control group with statistical significance（P〈0.05）.The ratio of CD4^＋ and DPT lymphocyte subtype in total T lymphocyte subtype of tuberculosis mice was significantly decreased（P〈0.05）.Conclusion The expression level IFN-γ was elevated in CD4^＋ T lymphocyte of tuberculosis mice.The expression level of IL-2 was depressed in CD4^＋ and DP T lymphocyte of tuberculosis mice.The phenomenon indicates that the antituberculosis immunity may start at early stage of tuberculosis infection but the nonspecific immunity is still predominant.

关 键 词：小鼠 结核病 T淋巴细胞亚群 γ-干扰素(IFN-γ) 白介素-2(IL-2) 

分 类 号：R52[医药卫生—内科学]