白藜芦醇对胃癌MGC-803细胞增殖抑制作用  

Inhibitive effect of resveratrol on proliferation of human gastric cancer MGC-803 cells

作　　者：郑丽维[1] 郑国华[1] 葛莉[1] 

机构地区：[1]福建中医学院护理学系护理学基础教研室,福州350108

出　　处：《中国公共卫生》2009年第12期1466-1467,共2页Chinese Journal of Public Health

摘　　要：目的探讨白藜芦醇对人胃癌MGC-803细胞株细胞周期影响及其机制。方法采用四甲基偶氮噻唑蓝(MTT)法和集落形成试验检测白藜芦醇对胃癌细胞的增殖抑制作用,流式细胞术检测细胞周期的改变,逆转录-聚合酶链式反应(RT-PCR)检测细胞凋亡抑制基因-2(bcl-2)、增殖基因c-myc的mRNA表达水平。结果白藜芦醇可抑制胃癌细胞增殖和集落形成能力,低、高剂量组的抑制率分别为6.9%,17.6%和27.98%,63.39%,差异均有统计学意义(P<0.05);白藜芦醇能使胃癌细胞周期阻滞于G0/G1期,明显下调胃癌细胞bcl-2、c-myc基因mRNA表达水平(P<0.05);用药24 h后,白藜芦醇高剂量组bcl-2和c-myc基因相对表达量分别为(0.54±0.04),(0.58±0.05),与空白对照组(0.91±0.05),(0.85±0.03)比较,差异均有统计学意义(P<0.05)。结论白藜芦醇可抑制胃癌MGC-803细胞增殖并使其细胞周期阻滞于G0/G1期,可能与下调bcl-2、c-myc基因表达有关。Objective To explore the effect and mechanism of resveratrol on cell cycle of human gastric cancer MGC-803 cells. Methods The blank control group, low dose resverstrol group （ 25 μg/mL） and high dose resverstrol group （50 μg/mL） were arranged in the experiment. After the administering of resverstrol to experimental groups for 24 hr, the inhibition rate of cell proliferation was measured with methyl-thiazolyl-tetrazolium method. The colony forming and the cell cycle were analyzed with flow cytometry, and the mRNA expression of bcl-2, c-myc gene were determined with RT-PCR method. Results Resveratrol could markedly inhibit proliferation of human gastric cancer cells compared with that of the blank control group. The cell cycle was blocked to G0/G1. The mRNA level of bcl-2 and c-myc gene were decreased compared with that of the blank control group. Condusion Resverstrol could inhibit cell proliferation and block the cell cycle to G0/G1 in human gastric cancer MGC-803 cells. The effects might be related to decreased expression of bcl-2 and c-myc gene.

关 键 词：白藜芦醇 胃癌MGC-803细胞株 细胞周期 胃癌细胞 

分 类 号：R931.71[医药卫生—生药学]