内毒素对小鼠肺、肝和心氧化损伤作用  

Endotoxin-induced oxidation damage in lung,liver and heart in mice

作　　者：解静芳[1] 崔宏莉[1] 金国文[1] 刘小娟[1] 郭晓君[1] 李瑞金[2] 

机构地区：[1]山西大学环境与资源学院,山西太原030006 [2]山西大学环境医学与毒理学研究所

出　　处：《中国公共卫生》2009年第12期1511-1512,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30740037;20677035)

摘　　要：目的探讨内毒素/脂多糖(LPS)对小鼠肺、肝、心氧化损伤的影响。方法24只ICR纯系雄性小鼠随机分为5,10,15 mg/kg 3个LPS染毒组和1个对照组,每组6只。采用腹腔注射方法进行LPS染毒,对照组注射生理盐水,测定染毒后肺、肝、心3种脏器中抗氧化物质和脂质过氧化(LPO)的水平。结果LPS腹腔注射引起所有受试脏器谷胱甘肽(GSH)含量下降,LPO水平升高,且随着LPS染毒剂量的增大,各器官组织氧化损伤程度加重。经t-检验,中、高剂量LPS处理组与对照组比较差异均有统计学意义。LPS对不同脏器引起的损伤程度不同,存在器官差异,总体上对肝、肺的损伤较为严重。结论LPS可引起小鼠肺、肝、心等多种脏器氧化损伤,且损伤程度与内毒素剂量成正相关。Objective To explore the oxidative damages caused by endotoxin（lipopolysaccharide,LPS） in lung,liver and heart in mice.Methods Twenty-four pure blood ICR mice were randomly divided into 4 groups.The treatment groups were injected intraperitoneally with LPS（5,10,15mg/kg） and the control group with normal saline.Then levels of glutathione（GSH） and lipid peroxidation（LPO） in lung,liver and heart of the mice were measured.Results LPS decreased the levels of GSH,but increased the levels of LPO in the lung,liver and heart and the oxidation damages of the organs increased with the increase of LPS dose.T-test showed that there were significant differences between LPS treatment groups（10mg/kg,15mg/kg） and the control group（P〈0.05）.The levels of oxidation damage caused by endotoxin were different in different organs,and the oxidation damage of liver,lung of treated groups were more serious.Conclusion Endotoxin can cause oxidation damage of lung,liver and heart in mice,and there is a positive correlation between the degree of damage and endotoxin dose.

关 键 词：内毒素 氧化损伤 还原型谷胱甘肽 脂质过氧化 

分 类 号：R996.1[医药卫生—毒理学]