不同发育阶段杉木人工林对土壤微生物群落结构的影响  

Effects of Cunninghamia lanceolata plantations at different developmental stages on soil microbial community structure

作　　者：刘丽[1,2,3] 段争虎[1] 汪思龙[2,3] 胡江春[2] 胡治刚[2] 张倩茹[2] 王书锦[2] 

机构地区：[1]中国科学院寒区旱区环境与工程研究所,兰州730000 [2]中国科学院沈阳应用生态研究所,沈阳110016 [3]中国科学院会同森林生态实验站,湖南会同418307

出　　处：《生态学杂志》2009年第12期2417-2423,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(40801093);中国科学院知识创新工程重要方向资助项目(KZCXZ-YW-405)

摘　　要：采用变性梯度凝胶电泳技术(DGGE),分析土壤细菌16SrDNA和土壤真菌28SrDNA特异性片段多态性,研究了不同发育阶段杉木人工林对土壤微生物群落结构的影响。结果表明:土壤微生物群落结构随着杉木人工林的发育年龄而改变,杉木人工林土壤微生物群落多样性和丰富度随杉木生长发育显著增加(P<0.05),但均显著低于次生阔叶林(P<0.05);聚类分析表明,不同发育阶段杉木人工林土壤真菌群落相似性均<60%,而土壤细菌群落相似性最高可达65%,由此可推测不同发育阶段杉木人工林土壤真菌群落结构变化较土壤细菌群落结构变化剧烈;相关性分析表明,不同发育阶段杉木人工林土壤速效氮、碳氮比与土壤微生物群落多样性显著相关(P<0.05)。本研究表明,长期种植单一杉木人工林能够通过改变土壤理化性质来影响土壤微生物群落组成,进而影响森林生态系统养分循环,导致人工林林分生产力下降。By the method of denaturing gradient gel electrophoresis（DGGE）,the polymorphism of soil bacterial 16S rRNA and fungal 28S rRNA specific fragments in 3-,11-,17-,21-,and 24-year old Cunninghamia lanceolata plantations was studied,with the effects of C.lanceolata plantation＇s development stage on soil microbial community structure analyzed.The Shannon diversity index（H）and richness（S）showed that the soil microbial community diversity increased significantly with the development of C.lanceolata plantation,but was still significantly lower than that in secondary broad-leaved forests（P0.05）.Cluster analysis（UPGMA）indicated that the soil fungal community similarity in different aged C.lanceolata plantations was less than 60%,whereas that of soil bacterial community similarity was up to 65%,suggesting that the shift of soil fungal community structure with the development of C.lanceolata plantation was more obvious than that of soil bacterial community structure.The soil microbial diversity had significant correlations with soil available N content and C/N ratio（P0.05）.This study indicated that long-term silviculture with pure plantations could affect soil microbial community via altering soil properties,which in turn,could affect the nutrient cycling in forest ecosystem and the plantation productivity.

关 键 词：杉木林 土壤微生物群落 16S RDNA 28S RDNA PCR-DGGE 

分 类 号：S714[农业科学—林学]