一氧化氮对强光胁迫下高羊茅叶片抗氧化系统的调节效应  

Regulation effect of nitric oxide on anti-oxidation system of tall fescue leaves under high light stress

作　　者：许岳飞[1] 金晶炜[2] 孙小玲[1] 周禾[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]中国农业大学资源与环境学院,北京100193

出　　处：《生态学杂志》2009年第12期2471-2476,共6页Chinese Journal of Ecology

基　　金：国家科技支撑计划资助项目(2006BAD16B09-2)

摘　　要：在强光胁迫下,采用水培法,使用外源NO和去除内源NO措施,研究了NO对2个高羊茅(Arid3和Houndog5)品种的抗氧化系统的调节作用。结果表明,Arid3的内源NO比对照增加201.5%,而Houndog5仅增加21.1%。Houndog5发生严重氧化损伤,电解质渗出率和丙二醛(MDA)含量分别比对照增加72.6%和85.1%,而Arid3受到的伤害较轻,分别比对照增加36.1%和30.1%。使用0.2mmol.L-1的NO专一清除剂2,4-羧基苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物(PTIO)清除内源NO,加剧强光胁迫对Arid3与Houndog5氧化伤害。应用0.1mmol.L-1的外源NO供体硝普钠(SNP)能降低强光胁迫造成的Arid3和Houndog5叶片脂氧合酶(LOX)活性,降低超氧自由基(O2.-)产生速率和质膜相对透性的增加以及MDA和H2O2的累积;同时,0.1mmol.L-1的SNP还能够诱导过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸氧化物酶(APX)和谷胱甘肽还原酶(GR)活性。而外源NO的保护作用被PTIO逆转。上述结果暗示,NO可能作为一个活性分子诱导抗氧化物酶的活性,减轻强光胁迫下2个高羊茅品种氧化损伤。By applying exogenous NO or depleting endogenous NO,this paper studied the regulation effect of NO on the leaf anti-oxidation system of tall fescue（Festuca arundinacea）cultivars Arid3 and Houndog5 in hydroponic culture under high light stress.Comparing with the control,the endogenous NO production under high light stress in Arid3 leaves was increased by 201.5%,while that in Houndog5 leaves was only increased by 21.1%.Houndog5 leaves were more liable in suffering oxidative damage than Arid3 leaves,with the electrolyte leakage and MDA content increased by 72.6% and 85.1%,and 36.1% and 30.1%,respectively.Pretreatment with 0.2 mmol·L-1 of NO eliminator 2-（4-carboxy-2-phenyl）-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide（PTIO）obviously aggravated the oxidative damage to Arid3 and Houdog5,while supplementing with 0.1 mmol·L-1 of NO donor sodium nitroprusside（SNP）alleviated the high light induced electrolyte leakage and malondiadehyde（MDA）and H 2O 2 contents in both Arid3 and Houndog5 leaves.The production of superoxide（O-· 2）was reduced,the activities of peroxidase（POD）,superoxide dismutase（SOD）,catalase（CAT）,ascorbate peroxidase（APX）and glutathione reductase（GR）were increased,and the lipoxygenase（LOX）activity was decreased.The effect of SNP was reverted when a NO-special scavenger PTIO was added.It was suggested that NO could effectively protect Arid3 and Houndog5 leaves from oxidative stress by increasing the activities of ROS-scavenging enzymes under high light stress.

关 键 词：一氧化氮 抗氧化酶 氧化损伤 强光胁迫 高羊茅 

分 类 号：S688.4[农业科学—观赏园艺]