蛋白沉淀-高效液相色谱法筛查血浆中61种常见的中枢神经系统药物  

Determination of 61 central nervous system drugs in plasma by protein precipitation-high performance liquid chromatography

作　　者：张吟[1,2] 陈崇宏[2] 林玲[3] 陈一农[1] 

机构地区：[1]福建医科大学第二临床医学院药剂科,福建泉州362000 [2]福建医科大学药学院药理学系,福建福州350004 [3]福建医科大学第二临床医学院免疫内科,福建泉州362000

出　　处：《色谱》2009年第6期787-793,共7页Chinese Journal of Chromatography

基　　金：福建省卫生厅青年课题(2007-1-27);泉州市科技局课题(2008Z24)

摘　　要：建立了利用蛋白沉淀提取血浆中61种常见的中枢神经系统药物并用高效液相色谱-二极管阵列检测器(HPLC-DAD)分析的方法。1mL血浆样品中加入1.5mL乙腈,旋涡混合后,离心,上清液过滤后直接采用HPLC测定。选用Agilent TC-C18色谱柱(250mm×4.6mm,5μm),以磷酸盐缓冲液和乙腈为流动相进行梯度洗脱,流速1.5mL/min,柱温35℃,检测波长210nm。61种药物的回收率均大于80%,相对标准偏差为0.94%～11.23%。采用乙腈沉淀蛋白,方法简便、快速、回收率高且稳定,能够作为系统毒物分析的通用前处理方法。该蛋白沉淀方法与HPLC-DAD技术结合,可应用于61种药物的分析。A method was established for the determination of 61 central nervous system drugs in plasma by using protein precipitation combined with high performance liquid chromatography-diode array detection （HPLC-DAD）. A volume of 1.5 mL acetonitrile was added into 1 mL plasma, after vortex, centrifugation and filtration, the supernatant was directly injected into HPLC. The separation was performed on an Agilent TC-C18 column （250 mm ×4.6 mm, 5 μm） with acetonitrile and phosphate buffer solution as mobile phase by gradient elution at a flow rate of 1. 5 mL/min. The detection wavelength was 210 nm; full spectra were recorded from 200 - 364 nm. The recoveries of 61 drugs were larger than 80% with the relative standard deviations （RSDs） ranged from 0.94% to 11. 23%. The protein precipitation method is simple, rapid, low-cost with good recoveries, reproducibility and suitable for the general pretreatment of the systematic toxicological analysis （STA） of the 61 drugs.

关 键 词：蛋白沉淀 高效液相色谱法 系统毒物分析 常见中枢神经系统药物 药物筛查 血浆 

分 类 号：O658[理学—分析化学]