免疫失败儿童乙肝病毒S基因“a”抗原决定簇序列分析  

Sequence analysis on HBV DNA "a" determinant mutation in children failed in HBIG vaccination

作　　者：虞勤[1] 刘玉琴[1] 王永忠[1] 江剑华[1] 

机构地区：[1]常州市第三人民医院妇产科,江苏常州213001

出　　处：《江苏大学学报（医学版）》2009年第6期502-504,共3页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:探讨乙肝疫苗联合高效价乙肝免疫球蛋白母婴阻断免疫失败儿童病毒S基因"a"决定簇的变异情况。方法:对30例经乙肝疫苗联合高效价乙肝免疫球蛋白母婴阻断免疫失败后血清表面抗原(HBsAg)阳性的儿童,采用聚合酶链反应方法(PCR)扩增其血清中HBV DNA S基因区,并对PCR产物直接标记测序。结果:30例HBsAg阳性儿童有28例血清HBV DNA阳性,其中有8例出现了S基因"a"决定簇的变异,变异率为28.6%,第126位的异亮氨酸(Ile)被苏氨酸(Thr)替代2例,第134位苯丙氨酸(Phe)被异亮氨酸替代2例,第145位甘氨酸(G ly)被丙氨酸(A la)替代4例。结论:乙肝疫苗联合高效价乙肝免疫球蛋白母婴阻断免疫失败儿童中存在S基因"a"抗原决定簇变异。Objective： To investigate hepatitis B virus（HBV） S gene ＂a＂ determinant mutation in children who failed to be protected by hepatitis B vaccine and hepatitis B immune globulin.Methods： HBV DNA S gene was amplified by polymerase chain reaction and sequenced directly. Results： There were 28 samples for HBV DNA positive in all children with HBsAg positive,eight mutations were detected,the mutation rate was 28.6%;two strain showed Ile-to-Thr at the 126 codon of surface antigen,two strain showed Phe-to-Ile at the 134 codon,and four strains showed Gly-to-Ala at the 145 condon of surface antigen. Conclusion： HBV S gene ＂a＂ determinant mutation existed in HBV vaccination and Hepatitis B immune globulin immune failure children.

关 键 词：乙型肝炎病毒 乙型肝炎疫苗 高效价乙肝免疫球蛋白 变异 基因 

分 类 号：R512.6[医药卫生—内科学]