斑点叉尾鮰病毒对几种淡水鱼类细胞系感染性的初步研究  

Study on Susceptibility of Multiple Fresh Water Fish Cell Lines to Channel Catfish Virus

作　　者：刘学芹[1,2] 王敏[1] 王卫民[1] Yuanan Lu 

机构地区：[1]华中衣业大学水产学院,湖北武汉430070 [2]Department of Public Health, University of Hawaii, Honolulu, HI 96822, USA

出　　处：《水生态学杂志》2009年第6期76-79,共4页Journal of Hydroecology

基　　金：教育部博士点新教师基金(20070504047);湖北省自然科学基金(2008CBB099);华中农业大学教师启动基金(2006XRC047)

摘　　要：研究斑点叉尾鮰病毒(Channel Catfish Virus,CCV)是否感染斑点叉尾鮰以外的鱼类,以评价CCV对我国珍稀、重要经济鱼类的潜在危害性。选择大眼鲑、草鱼、鲤、白鲟的细胞系为材料,从细胞病变、病毒增殖滴度等方面验证CCV对这些细胞系的感染性。实验表明,CCV对上述细胞系均可以感染,但细胞病变出现时间、病变程度和增殖滴度各有差异。其中,草鱼鳍条细胞系(GCF)、鲤上皮瘤细胞系(EPC)对CCV高度敏感。CCV在EPC中的增殖滴度与在斑点叉尾鮰卵巢细胞系(CCO)中相当,在GCF中的增殖滴度甚至高于CCO。研究结果表明,CCV除了感染CCO以外,还可以感染其它多种鱼类的细胞系,说明CCV病毒可能对斑点叉尾鮰以外的淡水鱼类具有感染性和潜在的危害性。To study the susceptibility of multiple fresh water fish cell lines to channel catfish virus （CCV） and to evaluate and assess potentially pernicious impact of CCV on several rare and important economic fish species in China. Five cell lines established from several fish species including grass carp fins （GCF）, epithelioma papulosum cyprini （ EPC）, white sturgeon spleen （ WSS - 2 ）, chinook salmon embryo （ CHSE - 214 ） and parental control channel catfish ovary （CCO） were tested and compared for their sensitivity to CCV infection. After virns infection, test cells were characterized by cell morphology, cytopathic effect （CPE） appearance time, and infectious virus titer. Our results indicated that all these cell lines were sensitive to CCV, but at varied levels. Comparative viral challenge assays revealed that, among these five fish cell lines, GCF exhibited to be the most sensitive one to CCV infection, and infectious viral particles produced in this cell line is significantly more than that generated from parental CCO cells. EPC cells were also highly susceptible to CCV infection with a similar level of viral production as compared to CCO. Experimental data showed that all the test five cell lines were susceptible to CCV and present study suggests that CCV can be a potential pathogen to infect gYass carp, Cyprinus carpio and other fresh water fishes.

关 键 词：斑点叉尾鮰病毒 感染性 细胞系 

分 类 号：S941.41[农业科学—水产养殖]