X线交错互补修复基因1和核苷酸剪切修复基因多态性与前列腺癌发病风险的相关性  

Association of genetic polymorphisms in the DNA repair gene XRCC1 and XPD with risk of prostate cancer

作　　者：刘杰[1] 宋宝[1] 王红[1] 田军[2] 陈阵[1] 石焕[1] 王哲海[1] 

机构地区：[1]山东省肿瘤防治研究院肿瘤内三科,济南250117 [2]安丘市人民医院肿瘤科

出　　处：《中华泌尿外科杂志》2009年第12期834-837,共4页Chinese Journal of Urology

摘　　要：目的探讨DNA损伤修复基因X线交错互补修复基因1（XRCC1）、核苷酸剪切修复基因（XPD）基因多态性与前列腺癌发病风险的关系。方法以358例前列腺癌患者和312例健康对照者为研究对象，采用聚合酶链反应-限制性片段长度多态技术检测XRCC1C26304T、G28152A和XPDA35931C位点基因型，以非条件logistic回归分析计算比值比（OR）及95％可信区间（CD，评估不同基因型与前列腺癌风险之间的相关性。结果前列腺癌组XRCC128152位点AA基因型者47例（13．1％），对照组24例（7．1％），携带此基因型者患前列腺癌风险显著增加（OR1．924，95％CI=1．126～3．288，P=0．017）。2组间XRCC1 C26304T和XPDA35931C位点基因型分布差异无统计学意义。3个基因位点联合分析结果显示，个体携带XRCC128152AA型及XPD35931AC＋CC型者发生前列腺癌风险显著增加（OR=3．087，95％CI1．081～8．813；OR=3．376，95％CI1．067～10．683；OR3．216，95％CI=1．439～7．188，P=0．004）。以患者年龄、PSA值、Gleason评分以及临床分期分层分析结果显示，携带XRCC128152AA及XPD35931Ac＋CC基因型者发病年龄明显低于携带野生基因型者（P〈0．05）。结论中国汉族人群XRCC1和XPD基因多态与前列腺癌发病有关，尤其是较年轻者。Objective To explore the relationship between DNA repair gene XRCC1 and XPD polymorphisms and individual susceptibility to prostate cancer. Methods PCR-restriction fragment length polymorphism assay was used to analyze the XRCC1 （C26304T and G28152A） and XPD A35931C polymorphisms in 358 prostate cancer patients and 312 healthy controls. Unconditional lo- gistic regression analysis was performed to calculate odd ratio （OR） and 95% confidence interval （CI） for estimating the correlation between different genotypes and prostate cancer risks. Results Fortyseven（13.1%） cases present XRCC1 28152AA genotype in prostate cancer group, while 24 cases in the control group （7.1%）, individuals with this genotype had a significantly increased risk for pros tate cancer （OR 1. 924, 95%CI=1. 126-3. 288, P=0. 017）. There was no significant difference between two groups at XRCC1 C26304T and XPD A35931C sites. Combined analysis of the three sites polymorphisms showed that individuals with XRCC1 28152 AA and XPD 35931AC＋CC genotype had a higher risk of prostate cancer than those with three wild genotypes （OR= 3. 087,95%CI 1. 081- 8.813;OR=3.376,95%CI 1. 067-10. 683 OR 3.216,95%CI=1.439-7.188,P=0.004）. Analysis stratified by age of onset, PSA, Gleason score and T stage revealed that XRCC1 28152AA and XPD 35931AC＋CC high-risk genotype was especially associated with early age at onset of prostate cancer （P〈0. 05）. Conclusions The XRCC1 and XPD genotypes may be contributed to the risk of developing prostate cancer, particularly for younger patients.

关 键 词：前列腺肿瘤 癌 DNA损伤修复基因 单核苷酸多态性 

分 类 号：R686[医药卫生—骨科学]