紫穗槐茎段愈伤组织诱导及再生体系的建立  

Induction of callus and plant regeneration for stem segments of Amorpha fruticosa

作　　者：管清杰[1] 罗秋香[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,黑龙江哈尔滨150040

出　　处：《辽宁林业科技》2009年第6期8-11,共4页Liaoning Forestry Science and Technology

基　　金：哈尔滨市青年基金(2007RFXN009);东北林业大学校基金(07049)

摘　　要：以紫穗槐茎段为研究对象,确定了影响其愈伤组织诱导的关键因子、不定芽分化及生根培养的最佳条件,建立了紫穗槐稳定高频再生体系。试验结果表明:紫穗槐茎段愈伤组织的最适诱导培养基为MS+6-BA4.0mg/L+NAA2.0mg/L+2,4-D0.5mg/L,诱导率为100%,经愈伤组织分化不定芽的最佳培养基为MS+6-BA0.5mg/L+NAA0.1mg/L+KT2mg/L,分化率为96.2%,增殖倍数为7.3;不定芽最佳生根培养基为1/2MS+酵母提取物0.5mg/L;再生植株移植在选用田园土、蛭石(5∶1)混合的基质上,光强3000lx,光照时间14h/d下生长,3周后成活率达85.67%。In this study, the stem segments of Amorphafruticosa are used as explants in tissue culture. The key factors for callus induction, together with the optimum medium for differentiation of adventitious bud and rooting culture are studied. An efficient regeneration system for stem segments of Amorphafruticosa was achieved at the same time. It indicated that MS＋6-BA 4.0mg/L＋ NAA 2.0mg/L ＋2,4-D 0.5 mg/L was the best medium for its callus induction, its induction rate was 100%; MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L＋KT 2mg/L was the optimum medium for differentiation of adventitious bud via callus, its differentiation rate was 96.2% and the coefficient of reproduction was 7.3. 1/2MS with yeast extraction 0.5mg/L was the optimum medium for adventitious bud. When the plant regeneration transplanted in garden soil mixed with vermiculite （the volume rate： garden soil/venniculite=5/1）, and the light intensity was 3 0001x, the photoperiod was 14 h/d, aider 3 weeks its survival percent was up to 85.67% .

关 键 词：紫穗槐 茎段 愈伤组织 再生体系 

分 类 号：S722.3[农业科学—林木遗传育种]