双亲灭活原生质体融合技术在苏云金杆菌菌种选育上的应用(Ⅱ)——融合产物的鉴定  

The Application of Inactived Protoplast Fusion to Bacillus Thuringiensis Ⅱ The Identification of Fusants

作　　者：陈五岭[1] 张芳琳[1] 景建洲[1] 孙连魁[1] 

机构地区：[1]西北大学生物学系

出　　处：《西北大学学报（自然科学版）》1998年第5期419-422,共4页Journal of Northwest University（Natural Science Edition）

基　　金：陕西省自然科学基金重点攻关项目资助

摘　　要：在双亲灭活原生质体融合选育苏云金杆菌新菌株的过程中，最终筛选出一株毒力效价较双亲分别提高４１％和１１７％的新菌株３＃。该菌株的酯酶同工酶谱及晶体蛋白多肽均与双亲有显著不同。A new Bt strain 3 # whose toxicity titer was 41% and 117% higher than those of its parents,has been obtained by inactived protoplast fusion technique.The estrase isoenzyme patterns,toxicity titer and polypeptides in parasporal crystals of fusants were quite different from that of their parental strains.

关 键 词：苏云金杆菌 酯酶同工酶 原生质体融合 育种 选育 

分 类 号：Q939.124[生物学—微生物学]