不同品系黑腹果蝇细胞系的建立(Ⅱ)——黑条体突变型(BSR)细胞系的建立和生物学特征  

Establishment of Variant Cell Line in Drosophila Melanogaster (Ⅱ) ——Establishment and Biological Character of Cell Line in Black streak body ·BSR Mutant

作　　者：曾庆韬[1] 钱远槐[1] 黄红[1] 严冰[1] 

机构地区：[1]湖北大学生命科学学院

出　　处：《湖北大学学报（自然科学版）》1998年第4期373-376,共4页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金;湖北省科委重点资助

摘　　要：为了广泛选择外源真核基因高频扩增及高效表达的载体和受体系统，利用改良的Ｍ３（ＢＦ）培养基从黑腹果蝇（ＤｒｏｓｏｐｈｉｌａＭｅｌａｎｏｇａｓｔｅｒ）黑条体（Ｂｌａｃｋｓｔｒｅａｋｂｏｄｙ·ＢＳＲ）的胚胎中建立了一株细胞系命名为ＢＳＲ９８０４．原代培养４０ｄ形成单层细胞，以１∶２分种率７ｄ传代一次，连续传代４０代次，细胞倍增时间为１８ｈ，细胞系以２倍染色体为主，未发现结构异常的染色体，２％胎牛血清为维持细胞生存的最小需要量，细胞系可在４℃普通冰箱中保存复苏．A cell line named BSR-9804 are established from embryos of Black streak body ·BSR in Drosophila Melanogaster by using improved M 3(BF) medium ,which help to select vector and acceptor system for exogenous eukaryon gene to amplify in high frequency and express with high efficiency Single layer cells were formed after primary culture 40 days, passage half cells every 7 days and continuous passage 40 times ,it took 18 hours to proliferate cells twice in number Cells of diploid chromosomes are prominant in the cell line, observed no abnormal chromosome 2% fetal bovine serum(FBS) is a mininal dose to maintain cells The cell line can be stored and resuscitated at 4℃ refrigerator

关 键 词：果蝇 黑腹果蝇 黑条体突变型 细胞系 细胞培养 

分 类 号：Q343.6[生物学—遗传学] Q782