用低龄动物制备高效价Et免疫血清的探讨  

Research on Preparation of High Titer Et Immune Serum by Low Age Animals

作　　者：朱壮春[1] 陈阳[1] 郝东升[2] 王艳茹[1] 马玉妹[1] 曹向可[1] 穆艮艮[1] 李金坤[1] 史相国[3] 

机构地区：[1]华北煤炭医学院生物科学系,河北唐山063000 [2]华北煤炭医学院附属医院,河北唐山063000 [3]河北省唐山陡河水库管理处,河北唐山063000

出　　处：《安徽农业科学》2009年第34期16861-16862,16896,共3页Journal of Anhui Agricultural Sciences

基　　金：唐山市科技局科技攻关项目(06125401A-3)

摘　　要：[目的]建立一种用低龄日本大耳白兔制备高效价迟钝爱德华氏菌(Edwardsiellatarda,Et)免疫血清的方法。[方法]先制备迟钝爱德华氏菌(Et)抗原。用日本大耳白兔作免疫动物,分2组试验,一组是2月龄组(T组),另一组是6月龄(S组)。采取耳缘静脉注射的方法,小剂量连续多次免疫日本大耳白兔,免疫后,收集免疫血清,然后用微量凝集反应法测定免疫血清抗体效价。[结果]凝集反应显示,在免疫剂量相同的情况下,第1次试血测Et免疫血清效价,S组的血清效价比T组的血清效价高,但在第2次试血测Et免疫血清效价时T组的血清效价与S组的血清效价一致。[结论]试验中所采用的T组动物(日本大耳白免)月龄较小,免疫后仍产生了与S组一致的高效价抗血清,说明用低龄日本大耳白兔制备高效价Et免疫血清的方法可行。[ Objective ]The study aimed to establish an approach that prepared high titer Et immune serum by low age Japanese rabbits. [ Method] Antigen of Et was prepared at first, taking Japanese rabbits as immunized animal, dividing two groups of experiments, one is two- month-old group （T group）, another is six-month-old group （S group）. Using auricular intravenous method, Japanese rabbits were continuous immunized with low-dose, then collecting immune sera. Immune serum antibody titer was determined with micro-agglutination reaction method. Result] Agglutination reaction showed that the Et serum titer of S group is higher than the T in the first testing with the same dose. But in the second testing, the serum titer of the T group and S group was consistent. [ Conclusion] The animals in the T group （Japanese rabbits） have smaller months, and produced high titer antiserum was consistent with S group, which indicated that the method of preparation of high titer Et immune serum by low age Japanese rabbits was feasible.

关 键 词：迟钝爱德华氏菌 免疫 免疫血清 日本大耳白兔 

分 类 号：S852.5[农业科学—基础兽医学]