稀土La^(3+)对Ba^(2+)胁迫下鄂尔多斯高原碱湖钝顶螺旋藻生长的影响  

Effects of Rare Earth La^(3+) on Growth of Spirulina in Alkaline Lake of Erdos Plateau under Ba^(2+) Stress

作　　者：巩东辉[1,2] 张少英[1] 乔辰[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]内蒙古科技大学生物技术研究所,内蒙古包头014010

出　　处：《安徽农业科学》2009年第34期17254-17255,17259,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30460104)

摘　　要：[目的]探讨稀土La3+对Ba2+毒害螺旋藻的生态生理效应的影响。[方法]以鄂尔多斯高原碱湖的钝顶螺旋藻(S1)为试材,采用生理与生物化学的方法研究了外源稀土元素La3+(10mg/ml)对重金属离子Ba2+胁迫(10mg/ml)下螺旋藻生长的影响,比较了施加La3+条件下螺旋藻对Ba2+的抗性效应以及二者拮抗作用随时间的变化。[结果]Ba2+对S1的生长量、叶绿素a和可溶性蛋白含量具有明显的抑制作用;Ba2+污染的培养液中添加一定浓度的La3+后,与单一重金属毒害相比,La3+可以缓解Ba2+导致的毒害作用,叶绿素a含量和可溶性蛋白、胞内多糖等生理指标有不同程度的上升;La3+对Ba2+具有较显著的拮抗效应。[结论]一定浓度的稀土La3+可有效地缓解Ba2+对螺旋藻生长的抑制,促进藻细胞叶绿素a含量、胞内多糖、可溶性蛋白等生理指标的上升。[ Objective ] The study aimed to discuss the effects of La3＋ on the ecological physiology of Spirulina poisoned by Ba2＋ [ Method ] With Spirulina platensis（ S1） in the alkaline lake of Erdos plateau as the tested material, the physiological and biochemical method were used to study the effects of the exogenous rare earth element La3＋ at 10 mg/ml on the growth of S1 in the alkaline lake of Erdos plateau under the heavy metal ion Ba2＋ stress （10 mg/ml） and compare the resistant effect of S1 on Ba2＋ and the change of both antagonistic effect with the time under adding La3 ＋. [ Result] The growth, the contents of the chlorophyl a and soluble protein were inhibited obviously under Ba2. stress. After adding La3＋ in the culture solution contaminated by Ba2＋ , compared with the poison by single heavy metal, La3＋ could relieve the damage caused by Ba2＋ stress, the growth of SL was improved and the physiological indexes such as contents of chlorophylla, intracellular polysaccharide and soluble protein were increased to different degree. The La3＋ had a remarkable antagonism effect on Ba2＋. [ Conclusion] The rare earth La3＋ at some concn. could effectively relieve the inhibition of Ba2＋ on the growth of S1, promote the rising of the physiological indexes such as contents of chlorophyll a in alga cells, intraeellular polysaeeharide and soluble protein.

关 键 词：螺旋藻 稀土 胁迫 

分 类 号：Q945.11[生物学—植物学]