MTT法测定8种中草药体外抗肝癌细胞SMMC-7721活性  

Determining on Activity of Anti-hepatocellular Carcinoma in vitro from Eight Chinese Traditional Medicine by MTT Method

作　　者：林董[1] 何爱明[1] 吴丽萍[2] 吴祖建[3] 

机构地区：[1]福建师范大学福清分校生化系,福建福清350300 [2]南昌大学生命科学学院,江西南昌330031 [3]福建农林大学植物保护学院,福建福州350001

出　　处：《安徽农业科学》2009年第34期17264-17266,共3页Journal of Anhui Agricultural Sciences

基　　金：福建省教育厅A类项目(JA08054)

摘　　要：[目的]建立一套适合于实验室高效、快速的体外抗肝癌活性物质筛选模型。[方法]通过MTT法,对芸香草、半边莲、蜜蒙花、甘草、地榆、柴胡、旱芹和莪术等8种中草药体外抗肝癌细胞SMMC-7721的活性进行初测;描绘肿瘤细胞生长曲线,倒置显微镜观察细胞不同阶段的生长状况。[结果]受活性物质作用细胞尽管仍然贴壁,但萎缩,边缘清晰化,与生长状况良好的细胞形态有明显区别;浓度为1.0～1.5mg/ml的甘草乙醇浸提液(ESCG)、莪术乙醇浸提液(ESCC)和柴胡乙醇浸提液(ESCB)对体外培养的肝癌细胞SMMC-7721有较强的抑制增殖作用,抑制率分别为51.6%、48.5%和52.9%,且抑制作用随着浓度的增加而加强。[结论]MTT法筛选可作为重要抗肿瘤的1个基本筛选模型。[ Objective ] The aim was to build up a set of efficient and rapid models for laboratory to screen anti-hepatocellular carcinoma active substance in vitro. [ Method] By using MTT method, the activities of anti-hepatocellular carcinoma SMMC-7721 in vitro from Cymbopogon distans ,Lobelia chinensis, Buddleja officinalis, Glycyrrhiza uralensis, Sanguisorba officinalis, Bupleurum chinense, Apium graveolen and Curuma zedoaria were tested. The growth curve of hepatoma cell was described, and the growth status in different periods were observed by inverted microscope. [ Result] Cells induced by active substance would be condensing,clear brim,which have significant differences from normal SMMC- 7721 cells. The results suggested that ESCG, ESCC, ESCB could inhibit proliferation of SMMC-7721 ceils at the concentration of 1.0 - 1.5 rag/ ml, and the inhibition rate were 51.6 % ,48.5 % ,52.9% respectively. With the increasing of concentration, the inhibition strengthened. [ Conclusion] MTT method could be used as a basic model for screening important antii-hepatoma.

关 键 词：MTT法 肝癌细胞SMMC-7721 筛选 抑制率 

分 类 号：S567.239[农业科学—中草药栽培]