透明质酸对体外培养大骨节病软骨细胞Ⅱ型胶原和聚集蛋白聚糖mRNA表达的影响  

Effect of hyluronic acid on collagen Ⅱ and aggrecan mRNA expression in Kashin-Beck disease chondrocytes cultured in vitro

作　　者：高宗强[1] 郭雄[2] 陈君长[1] 段琛[2] 马玮娟[2] 刘瑞宇[1] 顾其胜 

机构地区：[1]西安交通大学医学院第二附属医院骨三科,陕西省西安市710003 [2]西安交通大学医学院公共卫生系地方病研究所、环境与疾病相关基因教育部重点实验室,陕西省西安市710061 [3]上海其胜生物制剂有限公司,上海市201106

出　　处：《中国组织工程研究与临床康复》2009年第46期9061-9065,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：科技部国际合作重点专项(2006DFA33610);陕西省国际合作重点项目(2005KW-13);国家自然科学基金重点项目(30630058)~~

摘　　要：背景:透明质酸关节腔注射治疗骨关节炎已经成为一种常规方法,但对大骨节病的治疗还正在推广,施加透明质酸后对大骨节病患者软骨细胞代谢的影响,目前尚为空白。目的:课题创新性设计观察透明质酸对体外培养的大骨节病软骨细胞分泌Ⅱ型胶原和聚集蛋白聚糖的影响,以期能指导临床治疗。设计、时间及地点:对比观察,实验于2006-09/2009-01在西安交通大学医学院生物医学研究实验中心和地方病实验室完成。材料:大骨节关节病标本来源于6例大骨节关节病患者实施膝关节手术摘除的软骨及游离体,正常标本来自6例意外截肢或身亡的新鲜膝关节软骨。方法:分离、体外培养两组关节软骨细胞。两组细胞分别给予100,500mg/L不同剂量的透明质酸钠干预及阴性对照(未加干预),培养6d。主要观察指标:通过反转录-聚合酶链反应观察透明质酸对大骨节病和正常人软骨细胞Ⅱ型胶原和聚集蛋白聚糖mRNA表达的影响。结果:大骨节病组软骨细胞ColⅡ和aggrecan mRNA表达量均较正常对照降低(P<0.05),采用透明质酸钠补充治疗后,透明质酸钠100mg/L和500mg/L对大骨节病和正常人软骨细胞ColⅡ和aggrecan mRNA的表达均有促进作用,其中透明质酸钠500mg/L的促进作用更显著些。结论:补充透明质酸钠可以促进大骨节病软骨细胞ColⅡ和aggrecan的合成,其中,透明质酸钠500mg/L对大骨节病软骨细胞合成代谢的促进作用较透明质酸钠100mg/L明显。BACKGROUND： Intraarticular hyaluronic acid （HA） has been used as one common method in the treatment of osteoarthritis. But its application in treating Kashin-Beck disease （KBD） is generalizing. The effect of HA on chondrocyte metabolism in KBD patients remains unclear. OBJECTIVE： To investigate the effects of HA on the synthesis of collagen Ⅱ and aggrecan in KBD chondrocytes cultured in vitro to understand its clinical treatment for KBD. DESIGN, TIME AND SETTING： Comparative observation. The experiment was performed in the Laboratory Center of Biomedicine and Endemic Disease Laboratory of College of Medicine, Xi＇an Jiaotong University from September 2006 to January 2009. MATERIALS： Samples of KBD were from cartilage and corpus liberum excised from 6 KBD patients undergoing knee joint operation, and normal samples of fresh knee cartilages were provided by 6 people undergoing amputation or death by accident. METHODS： Chondrocytes of KBD and normal control were separated and cultured in vitro. Varying dosages of HA （100, 500 gm/L） were administered to normal and KBD chondrocytes cultured in vitro for 6 days. Negative control was not treated with HA. MAIN OUTCOME MEASURES： Effects of HA on collagen Ⅱand aggrecan mRNA expression in KBD patients and normal people by RT-PCR. RESULTS： Compared with normal control group, the mRNA level of collagen Ⅱand aggrecan were lower in KBD （P 〈 0.06）. After administration of HA （100, 500 mg/L） the mRNA level of collagen Ⅱand aggrecan were significantly increased, and the effects of 500 mg/L was more significant. CONCLUSION： HA can increase the collagen Ⅱ and aggrecan mRNA level of KBD chondrocytes cultured in vitro. In particular, 500 mg/L HA significantly promoted chondrocyte metabolism compared with 100 mg/L HA.

关 键 词：透明质酸 大骨节病 软骨细胞 细胞增殖 细胞凋亡 

分 类 号：R318[医药卫生—生物医学工程]