循环内皮祖细胞在阿托伐他汀钙干预肾性高血压大鼠体内的变化  

Effects of atorvastatin calcium on circulating endothelial progenitor cells in renovascular hypertensive rats

作　　者：杨松[1] 周建中[1] 

机构地区：[1]重庆医科大学附属第一医院心血管内科,重庆市400016

出　　处：《中国组织工程研究与临床康复》2009年第46期9133-9137,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:循环内皮祖细胞数量和功能降低预示高血压患者血管内皮修复能力降低,但有关他汀对高血压患者循环内皮祖细胞的影响尚不十分清楚。目的:血管内皮细胞及循环内皮祖细胞在阿托伐他汀钙干预肾性高血压大鼠体内的变化。设计、时间及地点:随机对照动物实验,2008-06/2009-02于重庆市神经病学重点实验室完成。材料:SPF级雄性SD大鼠24只,阿托伐他汀钙为辉瑞公司产品,批号:65837003。方法:随机抽取16只大鼠,应用经典的两肾一夹方法构建SD大鼠肾性高血压模型,随机分为高血压组、他汀组;剩余8只为对照组,行同样操作,但不套银夹。术后4周他汀组给予阿托伐他汀钙20mg/(kg?d)灌胃8周,其余两组大鼠给予等量蒸馏水灌胃8周。主要观察指标:实验第4,12周测各组大鼠血压、血脂,第12周通过苏木精-伊红染色观察胸主动脉内皮细胞层连续性,并测定循环内皮祖细胞数量及增殖、黏附和迁移能力。结果:他汀组第4,12周收缩压与同期模型组比较差异无显著性意义,但两组均明显高于同期对照组收缩压(P<0.01);他汀组大鼠血脂无明显变化。模型组大鼠胸主动脉内皮层连续性严重受损;他汀组大鼠血管内皮细胞受损明显减轻。模型组循环内皮祖细胞数量显著低于他汀组与对照组,差异有显著性意义(P<0.01);模型组循环内皮祖细胞增殖能力、黏附能力和迁移能力显著低于他汀组与对照组,他汀组仍低于对照组,3组之间比较差异有显著性意义(P<0.01)。结论:肾性高血压大鼠胸主动脉内皮细胞受损严重,循环内皮祖细胞数量及功能下降;阿托伐他汀钙可提高循环内皮祖细胞数量,改善循环内皮祖细胞功能。BACKGROUND： The quantity and function reduction of circulating endothelial progenitor cells （CEPCs） indicate that vascular endothelial repair capacity reduces in hypertension patients, but the impact of statins on the CEPCs is still not very clear. OBJECTIVE： To investigate the effects of atorvastatin calcium on endothelial cells and CEPCs in renovascular hypertensive rats DESIGN, TIME AND SETTING： Randomized controlled animal experiment was performed in Chongqing Key Laboratory of Neurology from June 2008 to February 2009. MATERIALS： Totally 24 SD male rats of SPF grade, and atorvastatin calcium was produced by Lipitor, Pfizer Inc. with batch number of 65837003. METHODS： The renovascular hypertension was induced with 2-kindey and 1-clip method in 16 SD rats, which were then randomly divided into two groups： hypertensive group （n=8） and statins group （n=8）. control group （n=8） underwent the same operation, without silver slip. At 4 weeks after model construction, statins group was treated with atorvastatin calcium 20 mg/（kg ~ d） for 8 weeks, given through lavage. Other two groups received equal volume of distilled water for 8 weeks. MAIN OUTCOME MEASURES： At 4 and 12 weeks of the experiment, three groups of rats were measured blood pressure and blood lipids; at 12 weeks, the thoracic aortic endothelial cells continuity was investigated through hematoxylin-eosin stain; the quantity, proliferation, adhesion and migration capacity of CEPCs were determined. RESULTS： At 4 and 12 weeks of the experiment, there was no significant differences in the systolic pressure between statins group group and model group, which were dramatically high than control group （P 〈 0.01）; the blood lipids in the statins group was not changed. In the model group, rat aortic endothelial cells were greatly damaged; the injury of CEPCs was dramatically relieved in the model group. Compared with statins group and control group, the number of CEPCs was significantly decreased, the capacities of prolifera

关 键 词：循环内皮祖细胞 阿托伐他汀钙 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]