Fine mapping and cloning of MT1,a novel allele of D10  

作　　者：Yong Zhou Jinyan Zhu Zhengyi Li Fei Gu Honggen Zhang Shuzhu Tang Minghong Gu Guohua Liang 

机构地区：[1]Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China

出　　处：《Progress in Natural Science:Materials International》2009年第12期1683-1689,共7页自然科学进展·国际材料（英文版）

基　　金：supported by the State Key Program of Basic Research of China(Grant Nos.2005CB120807 and 2006CB101703);the National Natural Science Foundation of China(Grant No.30530118);the Key Program of the Bureau of Education of Jiangsu Province(Grant No. 05KAJ2012).

摘　　要：Rice tillering is an important determinant for grain production. To investigate the mechanism of tillering, we characterized a multiple tillering mutant （mt1） identified from the japonica variety, Zhonghua 11, treated with EMS. This mutant exhibits advanced tillering development and dwarfed compared with wild-type plants. Genetic analysis and fine gene mapping indicated that the mt1 mutant was controlled by a recessive gene, residing on a 29-kb window on AP003376 of chromosome 1. One putative gene in this region, encoding a carotenoid cleavage dioxygenase 8 （CCD8）, was allelic to DIO. The mt1 mutant phenotype was complemented by introduction of wild-type MT1, and knockdown of MT1 in wild-type rice mimicked the mutant phenotype. Real-time PCR analysis indicated that the MT1 gene is expressed highly in stems and at a low level in axillary buds, panicles, leaves, and roots. In addition, MT1 expression is clearly under feedback regulation.瑞斯是为谷物生产的一个重要决定因素到 ering 为止。调查机制到 ering 为止，我们描绘了一到从装饰用的梨树变化识别的 ering 异种(mt1 ) 为止多重， Zhonghua 11，与他们对待。展览推进了到 ering 开发为止并且与野类型的植物相比相形见绌的这异种。印射的基因分析和好基因显示 mt1 异种被后退的基因控制，住在染色体 1 的 AP003376 上的一扇 29-kb 窗户上。在这个区域的一通常认为的基因，编码类胡萝卜素劈开 dioxygenase 8 (CCD8 ) ，对 D10 突变而产生之遗传。mt1 异种显型被野类型的 MT1 的介绍补充，并且在模仿的野类型的米饭的 MT1 击倒变异的显型。实时 PCR 分析显示 MT1 基因在腋的芽，圆锥花序，叶子，和根在茎并且在底层高度被表示。另外， MT1 表示清楚地在反馈规定下面。

关 键 词：RICE TILLERING Map-based cloning： OsCCD8 Feedback regulation 

分 类 号：S511[农业科学—作物学] Q347[生物学—遗传学]