大鼠脑干胶质瘤模型的建立  

Establishment of C6 rat brainstem glioma model

作　　者：王宇[1] 田永吉[1] 李德志[1] 万虹[2] 历俊华[2] 王军梅[2] 张月清[2] 万伟庆[1] 李大鹏[1] 武文浩[1] 阴鲁鑫[1] 张力伟[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,100050 [2]首都医科大学附属北京神经外科研究所

出　　处：《中华神经外科杂志》2009年第11期1044-1046,共3页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（30772237）

摘　　要：目的建立大鼠脑干胶质瘤模型，为脑干胶质瘤的研究提供动物实验平台。方法将1×10^6个大鼠C6胶质瘤细胞通过立体定向头架注射到大鼠脑桥，种植后2周对大鼠行磁共振扫描观察，然后行灌流固定取脑，HE染色。结果磁共振检查均发现肿瘤生长，肿瘤为长T1长T2信号，经大鼠舌静脉注射Gd—DTPA信号明显增强。HE染色显示肿瘤为胶质母细胞瘤，呈浸润性生长，瘤内新生血管丰富，假栅栏样坏死明显。结论大鼠C6胶质瘤细胞株可以建立脑干胶质瘤模型，成瘤率高，重复性好。Objective To establish C6 rat brainstem glioma model to provide a platform of animal experiment for the research of brainstem gliomas. Method 1 ×10^6 C6 glioma cells were injected stereotactically into rat brainstem （pons） exactly. 2 weeks after implantation, MRI scan was performed to observe the growth of tumors, then the rats with tumors were perfused with 4% paraformaldehyde and the brainstems were sectioned and inspected. The sections were stained by H＆E. Results Tumors were found in the brainstems which were confirmed by MRI scan with a low T1 and high T2 signals, and were enhanced obviously. There was no extracranial growth of tumor. H＆E stains confirmed that the tumor was gliobtastoma. The growth was invasive,the new vessels were plentiful, and the palisade - like necrosis was obvious. Conclusions C6 rat brainstem glioma model can be established safely, steadily and repeatedly with a high tumorigenic rate, which can be confirmed by pathology and MRI scan.

关 键 词：脑干 神经胶质瘤 动物模型 磁共振 

分 类 号：R686[医药卫生—骨科学]