Mining cotton fiber strength candidate genes based on transcriptome mapping  

作　　者：LIU HengWei WANG XingFen PAN YuXin SHI RuiFeng ZHANG GuiYin MA ZhiYing 

机构地区：[1]Key Laboratory of Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding 071001, China [2]College of Life Science, Yangtze University, Jingzhou 434025, China

出　　处：《Chinese Science Bulletin》2009年第24期4651-4657,共7页

基　　金：Supported by the National Basic Research Program of China (Grant No. 2010CB126000);National High Technology Research and Development Program of China (Grant No. 2006011001044);the National Natural Science Foundation of China (Grant Nos. 30671322 and 30871561)

摘　　要：Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.Cotton fiber strength is mainly determined during the secondary cell wall （SCW） thickening stage. In 24―25 days post anthesis （DPA） of SCW thickening stage, cDNA-amplified fragment length polymorphism （AFLP） was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping （CIM） through quantitative trait locus （QTL） scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments （TDFs） from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans （cM）. Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.

关 键 词：纤维比强度 基因转录 扩增片段长度多态性 棉花 映射 候选人 数量性状位点 复合区间作图法 

分 类 号：S562.032[农业科学—作物学] Q756[生物学—分子生物学]