The LTR of endogenous retrovirus ev21 retains promoter activity and exhibits tissue specific transcription in chicken  

作　　者：LU XiaoQing HAN JinRun LIU XiaoFang LIN TongHui LI YuLin HU XiaoXiang LI Ning 

机构地区：[1]State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China [2]College of Animal Science & Technology, China Agricultural University, Beijing 100193, China

出　　处：《Chinese Science Bulletin》2009年第24期4664-4670,共7页

基　　金：Supported by the National Basic Research Program of China (Grant No. G20000161);National Natural Science Foundation of China (Grant No. 3022804)

摘　　要：Endogenous viruses integrate into the host genome and influence the expression of neighboring genes through their long terminal repeats (LTRs). In this study, we analyzed the promoter, enhancer and transcriptional activities of the chicken endogenous retrovirus ev21 LTR. The LTR was cloned into pGL3-basic and pGL3-promoter luciferase vectors in forward and reverse orientation separately. The luciferase activities were detected respectively in chicken embryonic fibroblast cell, human embryonic kidney cell line, human lung carcinoma cell line, Chinese hamster ovary cell line, and murine melanoma cell line. Relative luciferase activity analysis indicated that the ev21 LTR retained bi-directional promoter activity but no detectable enhancer activity in these cells. The constructs containing the LTR and F1 region show stronger promoter activity than the constructs containing only LTR. The transcriptional pattern of ev21 LTR varied in tissues of late feathering White Leghorn chicken at post-hatch day one. Skin exhibits the highest expression in the tissues examed. Collectively, our results indicate that the ev21 LTR exhibits tissue-type specific expression in White Leghorn chicken, and it also have regulatory potential.Endogenous viruses integrate into the host genome and influence the expression of neighboring genes through their long terminal repeats （LTRs）. In this study, we analyzed the promoter, enhancer and transcriptional activities of the chicken endogenous retrovirus ev21 LTR. The LTR was cloned into pGL3-basic and pGL3-promoter luciferase vectors in forward and reverse orientation separately. The luciferase activities were detected respectively in chicken embryonic fibroblast cell, human embryonic kidney cell line, human lung carcinoma cell line, Chinese hamster ovary cell line, and murine melanoma cell line. Relative luciferase activity analysis indicated that the ev21 LTR retained bi-directional promoter activity but no detectable enhancer activity in these cells. The constructs containing the LTR and F1 region show stronger promoter activity than the constructs containing only LTR. The transcriptional pattern of ev21 LTR varied in tissues of late feathering White Leghorn chicken at post-hatch day one. Skin exhibits the highest expression in the tissues examed. Collectively, our results indicate that the ev21 LTR exhibits tissue-type specific expression in White Leghorn chicken, and it also have regulatory potential.

关 键 词：内源性逆转录病毒 启动子活性 白来航鸡 组织特异性 展品 铁 胚胎成纤维细胞 宿主基因组 

分 类 号：Q959.483[生物学—动物学] S858.28[农业科学—临床兽医学]