Overexpression of the stress-induced OsWRKY08 improves osmotic stress tolerance in Arabidopsis  

作　　者：SONG Yu JING ShaoJuan YU DiQiu 

机构地区：[1]Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Chinese Science Bulletin》2009年第24期4671-4678,共8页

基　　金：Supported by the Knowledge Innovation Project of The Chinese Academy of Sci-ences (Grant No. KSCX2-YW-N-007);National Natural Science Foundation of China (Grant No. 30370803);National Science and Technology Infrastructure Pro-gram of the Ministry of Science and Technology of China (Grant No. 2005DKA21006);the "100 Talents Project" of Chinese Academy of Sciences

摘　　要：Previous Northern blotting analyses of rice seedlings have screened several WRKY genes among the transcripts that are differentially regulated in the following treatments: high salinity, cold stress, polyethylene glycol (PEG) and heat shock. Here, we report characterization of a WRKY gene, OsWRKY08, in rice, which was found to be inducible by PEG, NaCl, Abscisic acid (ABA), and naphthalene acetic acid (NAA) as its ortholog AtWRKY28 in Arabidopsis. To determine whether overexpression of OsWRKY08 alters abiotic stress tolerance, 35S::OsWRKY08 recombinant was generated and transformed into Arabidopsis. Physiological tests indicated that 35S::OsWRKY08 transgenic Arabidopsis displayed increased tolerance to mannitol stress through increasing the lateral root number and primary root length during seeding root development. Further, semi-quantitative RT-PCR showed that AtCOR47 and AtRD21, two ABA-independent abiotic stress responded genes, were induced in 35S::OsWRKY08 transgenic plants. These results suggest OsWRKY08 improves the osmotic stress tolerance of transgenic Arabidopsis through an ABA-independent signaling pathway.Previous Northern blotting analyses of rice seedlings have screened several WRKY genes among the transcripts that are differentially regulated in the following treatments： high salinity, cold stress, polyethylene glycol （PEG） and heat shock. Here, we report characterization of a WRKY gene, OsWRKY08, in rice, which was found to be inducible by PEG, NaCl, Abscisic acid （ABA）, and naphthalene acetic acid （NAA） as its ortholog AtWRKY28 in Arabidopsis. To determine whether overexpression of OsWRKY08 alters abiotic stress tolerance, 35S：：OsWRKY08 recombinant was generated and transformed into Arabidopsis. Physiological tests indicated that 35S：：OsWRKY08 transgenic Arabidopsis displayed increased tolerance to mannitol stress through increasing the lateral root number and primary root length during seeding root development. Further, semi-quantitative RT-PCR showed that AtCOR47 and AtRD21, two ABA-independent abiotic stress responded genes, were induced in 35S：：OsWRKY08 transgenic plants. These results suggest OsWRKY08 improves the osmotic stress tolerance of transgenic Arabidopsis through an ABA-independent signaling pathway.

关 键 词：转基因拟南芥 渗透胁迫 应力诱发 NORTHERN杂交 35S启动子 非生物胁迫 水稻幼苗 WRKY 

分 类 号：Q943.2[生物学—植物学] Q945.78