四川阿坝大骨节病病区粮食和T2毒素对大鼠骨关节发育的影响  

Compare study of rat bone and joint development effected by T2 toxin and KBD-affected feed of epidemic district in the A'ba Autonomous region of the P.R.China

作　　者：康鹏德[1] 颜登鲁[1] 姚运峰[1] 李兴波[1] 杨静[1] 沈彬[1] 周宗科[1] 裴福兴[1] 

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国骨与关节外科》2009年第5期404-410,共7页Chinese Journal of Bone and Joint Surgery

基　　金：十一五国家科技支撑计划项目(2007BA125B04)

摘　　要：目的探讨四川阿坝大骨节病(Kaschin-Beck disease,KBD)病区粮食(病粮)和T2毒素干预对大鼠骨关节发育的影响。方法78只断乳的Wistar大鼠,雌雄各半,体重60～65 g。随机分为3组:A组(24只)为病粮饲料喂养;B组(30只)为普通饲料+T2毒素喂养;C组(24只)为普通饲料喂养。分组喂养4周后,组织标本行HE和Masson染色。结果A组和C组在喂养中无死亡,B组有12只死亡。A组大鼠毛发色泽暗,活动较少,体重每周增加8～12g,平均(10.3±2.1)g;B组大鼠毛发色泽暗,活动少,体重每周增加12～16g,平均(14.3±2.1)g;C组大鼠毛发色泽光亮,活动如常,体重每周增加15～20 g,平均(17.7±3.5)g。A组体重增加较B组和C组少(P<0.05);B组体重增加较C组少(P<0.05)。第1周时,A组和B组的骺板在骺板各细胞带均无坏死;第2周时,A组有1只、B组有2只出现肥大细胞带灶状坏死;第4周时,A组有7只、B组有6只出现肥大细胞带灶状坏死和片状坏死,其中A组有2只、B组有3只出现增殖细胞带灶状坏死。C组在第1～4周均未出现坏死。A组和B组骺板胶原Masson染色较C组明显减弱,染色不均匀,软骨细胞坏死区胶原染色消失。第1、2周时,各组大鼠干骺端骨小梁排列整齐,无明显差异;第4周时,C组干骺端骨小梁排列整齐、紧密,B组和A组干骺端均出现较多的成纤维细胞,骨化线不整齐,A组较B组明显。结论病粮和T2毒素干预下大鼠骺板软骨细胞出现灶状及片状坏死,干骺端均出现较多的成纤维细胞,骨化线不整齐;且病粮较T2毒素干预明显,提示病粮中有其他致病因素存在。Objective To investigate the pathogenesy and epiphyseal plate chondrocyte necrosis feature of the Kash-in-Beck disease （KBD） rat model with T2 toxin and KBD-affected feed of epidemic district in the A＇ba autonomous region of China. Methods Seventy eight ablactation Wistal rat （59 male and 39 female） weighing about 65 g were obtained from Sichuan Medical Center （Chengdu, China）, and randomly divided into 3 groups （group A, B and C）. In group A, 24 rats were fed with KBD-affected feed; in group B, 30 rats were fed with commercial rats＇ feed and T2 toxin by intragastric administration ; and in group C, 24 rats were fed with commercial rats＇ feed only. The histological sections were stained with hematoxylin and eosin and Masson. Results No rat died in groups A and C, but 12 rats died in group B. The rats in group C gained the most weight （ P 〈 O. 05 ）, followed by group B （ P 〈 O. 05 ） and group A （ P 〈 O. 05 ）. One rat had chondrocyte focus necrosis at labrocyte cell zonal at week 2 in group A. At week 4, 7 rat had chondrocyte focus necrosis at labrocyte cell zonal. Two rats had focus necrosis at proliferatin cell zonal, 3 rats had lamellar necrosis at labrocyte cell zonal, 4 rats had focus necrosis at labrocyte cell zonal, and 2 rats had penetration necrosis. Two rats had chondrocyte focus necrosis at labrocyte cell zonal at second week in B group. At forth week, 6 rat had chondrocyte focus or lamellar necrosis at labrocyte cell zonal. Three rats had focus nec.rosis at proliferatin cell zonal, and 3 rats had penetration necrosis. There were no epiphyseal plate chondrocyte necroses in group C at weekl, 2, and 4. The epiphyseal plate Masson dye of group C showed deep blue color of collogen, and in group A and group B collogen was damp blue color, and the drum dyeing was uneven, and the collogen was decoloration on the necrosis district. Conclusion With T2 toxin and KBD-affected feed intervention, focus necrosis and lamellar necrosis at epiphyseal plate appear in KBD rat. KBD-af

关 键 词：大骨节病 T2毒素 大骨节病病区粮食 阿坝地区 

分 类 号：R684.1[医药卫生—骨科学]