正交试验优化银杏胚愈伤组织继代培养及黄酮积累  

Optimizes the Callus Growth and Flavonoids Formation in Embryo of Ginkgo biloba L. by Orthogonal Test

作　　者：胡燕梅[1] 张治华[1] 周骏江[1] 梅双喜[1] 宋鹏飞[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415

出　　处：《北方园艺》2009年第12期34-37,共4页Northern Horticulture

基　　金：湖北省教育厅科学技术研究资助项目(B20094008);武汉市高校科学研究资助项目(2008K079)

摘　　要：采用正交试验研究了基本培养基、NAA、6-BA、植酸(PA)4因素各3水平对银杏胚愈伤组织继代培养及黄酮积累的影响,并测定了愈伤组织生长曲线。结果表明:银杏胚愈伤组织继代培养的最佳组合是N6+NAA3.0mg/L+6-BA2.0mg/L+0.1%PA,各因子影响程度为基本培养基>NAA>6-BA>PA;愈伤组织黄酮积累量最佳组合是B5+NAA0.1mg/L+6-BA0.5mg/L+0.05%PA,各因子影响程度为NAA>PA>6-BA>基本培养基。愈伤组织生长曲线基本呈"S"型,黄酮积累的适宜培养周期为40～50d。The effects of different concentration and combinatibns of NAA, 6-BA, PA and minimal medium on embryo a callus of Ginkgo biloba L. for subculture and flavonoids formation were studied by using orthogonal test,and combinations of optimum treatment were selected. The results showed.the optimum combinations were N6 ＋NAA 3. 0 mg/L＋ 6-BA 2. 0 rag/L＋0. 1%PA for embryo callus subculture and effect sequence of four effects was minimal medium〉 NAA〉6-BA〉PA; the optimum combinations were B5 ＋NAA 0. 1mg/L＋6-BA 0. 5 mg/L＋0. 05%PA for flavonoids formation and effect sequence of four effects was NAA〉PA〉6-BA〉minimal medium. Growth curve of callus of Ginkgo biloba L. was curve ＂S＂ and the optimum culture cycle of flavonoid content was 40-50 d.

关 键 词：银杏 正交试验 愈伤组织 黄酮 植酸 

分 类 号：S664.303.6[农业科学—果树学]