烟草赤星病菌拮抗菌发酵液的防病作用  

Resistance of the zymotic fluid of antagonistic actinomyces against Alternaria alternata

作　　者：余淑英[1] 黎定军[2] 彭曙光 何莲[1] 欧晓明[1] 

机构地区：[1]湖南化工研究院国家农药创制工程技术研究中心,湖南长沙410007 [2]湖南农业大学生物安全科学技术学院,湖南长沙410128 [3]湖南省烟草专卖局,湖南长沙410014

出　　处：《湖南农业大学学报（自然科学版）》2009年第6期664-667,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南农业大学稳定人才基金项目(50262-62030204008)

摘　　要：以拮抗烟草赤星病菌的两株放线菌SZF2和SZF7为材料,对其发酵液防赤星病菌的效果及机理进行了初步研究.结果表明,SZF2和SZF7的无菌发酵液能抑制烟草赤星病原菌孢子萌发,同时还能激发烟草产生过量过氧化物酶(POD)和多酚氧化酶(PPO),从而提高烟草对赤星病菌的抗性.离体和盆栽试验结果表明,SZF2和SZF7的发酵液可明显降低烟草赤星病的病情指数,其盆栽试验相对防效分别为48.94%和50.13%.The zymotic fluid of two antagonistic actinomycetes strains, SZF2 and SZF7, showed strong antagonistic activity against Alternaria alternata. The control efficiency and antagonistic mechanism of the zymotic fluid have been preceded, whose results suggested that the zymotic fluid of SZF2 and SZF7 not only inhibited spore germination, but also increased activities levels of peroxidase（POD） and polyphenol oxidase（PPO） of tobacco plant. We induced these properties to give an ability to against Alternaria alternate. Meanwhile, we inoculated detached leaves and irrigated 13-leaves-old tobacco plants with the zymotic fluid of two strains respectively. Results showed that the the zymotic fluid could reduce the disease index of Alternaria alternate, and in a pot experiment, the control efficiency of SZF2 and SZF7 was 48.94% and 50.13%, respectively.

关 键 词：烟草赤星病 拮抗放线菌 生物防治 

分 类 号：S482.28[农业科学—农药学]