金黄色葡萄球菌肠毒素A对H22小鼠移植瘤的抑制作用  

The Inhibition Effect of Staphylococcal Enterotoxin A on H22 Solid Tumors in Mice

作　　者：王雪[1] 孙嘉琳[1] 张云[1] 冯艳敏[1] 陈自辉[1] 李政楠[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《生物技术通报》2009年第12期115-118,共4页Biotechnology Bulletin

基　　金：天津市科委资助应用基础研究重点项目(06YFJZJC02600)

摘　　要：旨在探讨金黄色葡萄球菌肠毒素A(SEA)在KM鼠体内的抑瘤效果。建立H22荷瘤小鼠模型,将20只小鼠随机分为对照组(生理盐水)、低剂量组(15μg/ml)、中剂量组(25μg/ml)和高剂量组(50μg/ml),观察肿瘤生长趋势,计算抑瘤率,通过ELISA检测IL-2的含量。结果显示,给药组肿瘤生长趋势均较对照组缓慢;对照组、低剂量组、中剂量组、高剂量组的抑瘤率分别为0,28.9%,34.0%,51.0%(P<0.05);血清中IL-2含量分别为58.9pg/ml、83.6pg/ml、91.8pg/ml、118.1pg/ml。金黄色葡萄球菌肠毒素A(SEA)可抑制H22肿瘤的生长,并上调IL-2的分泌。It was to study inhibition effect of staphylococcal enterotoxin A（SEA） on H22 solid tumors in KM mice. A total of 30 cases of S180 mice were randomly assigned into 4 groups, control（saline） , low doses （ 15μg/ml）, middle doses （25 μg/ml ）, and high doses（50 μg/ml）. The inhibition ratio of tumor growth and value of IL-2 in serum were studied respectively. Results showed that compared to the control group, the tumor growth of the treatment groups was slowly. The tumor-inhibition ratios of control, low doses, middle doses,and high doses were 0,34. 0% ,28. 9% and 51.0% （P 〈0. 05）and the values of IL-2 were 58.9 pg/ml,83.6 pg/ml,91.8 pg/ ml,and 118.1 pg/ml. Therefore, these results suggest that SEA can inhibit the growth of H22 solid tumors in vivo,and increase the secretion of IL-2.

关 键 词：金黄色葡萄球菌肠毒素A(SEA) ELISA IL-2 抑瘤作用 

分 类 号：R73-3[医药卫生—肿瘤]