青蒿素对人乳腺癌细胞MDA-MB-435辐照后微核的影响研究  

The effect of artemisinin additive combined with irradiation on micronucleus of human breast cancer cells MDA-MB-435

作　　者：刘杨[1,2] 曹建平[1] 樊赛军[1] 朱巍[1] 彭晓梅 唐艳[1] 陈遐林[1] 吴锦昌 周俊东 

机构地区：[1]苏州大学放射与公共卫生学院江苏省放射医学与防护重点实验室,苏州215123 [2]苏州市肿瘤诊疗中心实验室,苏州215000 [3]苏州市吴江疾病预防控制中心,苏州215200

出　　处：《辐射研究与辐射工艺学报》2009年第6期365-368,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(30670632);长江学者创新团队发展计划(IRT0849)资助

摘　　要：本实验主要分析青蒿素作用后p53功能突变的人乳腺癌细胞MDA-MB-435微核的变化。通过MTT法测定不同药物浓度及不同药物作用时间对细胞毒性的影响,通过胞质分裂阻滞法(CB法)计数青蒿素作用联合不同剂量60Coγ射线照射后细胞的微核率(Micronucleus frequency,MNF)和微核细胞率(Micronucleus cell frequency,MNCF),将青蒿素作用的细胞与单纯照射细胞的微核率和微核细胞率的统计分析结果进行比较。实验结果表明,青蒿素对实验细胞MDA-MB-435毒性较小,在实验药物浓度为200μmol/L,作用时间为24h时,将CB微核法得到的数据进行回归拟合可以得到药物作用及未经药物作用的剂量效应曲线,比较两组曲线,青蒿素作用组的微核率及微核细胞率明显高于单纯照射细胞(p>0.05)。In order to analyze the change of micronucleus in the p53 mutant human breast cancer cells MDA-MB-435 caused by artemisinin additive, MTr method was used to check the effect of different concentration and treatment time of artemisinin to cytotoxicity. Cytokinesis block micronucleus method （CB method） was used to determine the micronucleus frequency （MNF） and the micronucleus cell frequency （MNCF） of cells treated by artemisinin additive and with different doses of ^60Co γ-rays. The experiment results show that artemisinin is of little toxicity to MDA-MB-435. When drug concentration is 200μmol/L and treatment time is 24 h, the dose-response curves of MNF and MNCF of irradiated cells combined with treatment of or not artemisinin additive confirm that MNF and MNCF of cells treated by artemisinin are both significantly higher than that treated by radiation alone. It can be deduced that artemisinin may have some sensitive action on radiation therapy.

关 键 词：青蒿素 人乳腺癌细胞 微核 

分 类 号：R811.5[医药卫生—放射医学] R394.7[医药卫生—临床医学]