HPLC法测定不同产地刺五加叶中绿原酸和金丝桃苷含量  

Determination of Chlorogenic Acid and Hyperoside in Leaves of Acanthopanax Senticosus Produced in Different Areas by HPLC

作　　者：邵璟[1] 狄留庆[1] 谢新[1] 董宇[1] 赵晓莉[1] 吴灏昕[1] 刘产明[2] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]南京中医药大学附属常州市中医院,江苏常州213003

出　　处：《中国中医药信息杂志》2009年第10期41-43,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立刺五加叶中绿原酸及金丝桃苷的含量测定方法,测定不同产地刺五加叶中绿原酸和金丝桃苷的含量。方法采用Hedera?ODS-2 C18柱(250 mm×4.6 mm,5μm),乙腈-0.4%磷酸(17∶83)为流动相,检测波长为360 nm。结果绿原酸和金丝桃苷质量浓度分别在10.2～306μg/mL(r=0.999 5)和10.4～312μg/mL(r=0.999 8)之间,与峰面积均呈良好的线性关系;平均回收率分别为97.14%和98.21%,RSD分别为2.65%和1.89%。不同产地样品中所含的量相差较大。结论本法操作简便、准确、具有良好的重复性,为刺五加叶的进一步开发应用及质量控制奠定了基础。Objective To establish a HPLC method for the content determination of Chlorogenic acid and Hyperoside in the leaves of acanthopanax senticosus from different areas.Methods The samples were separated on Hedera？ ODS-2 C18 column,using acetonitrile-0.4% phosphoric acid（17∶83） as mobile phase,the detection wavelength was 360 nm.Result Chlorogenic acid and Hyperoside showed good linear relationship over the ranges of 10.2～306 μg/mL（r =0.999 5） and 10.4～312 μg/mL（r =0.999 8）, respectively. The average recoveries were 97.14% and 98.21% with RSD at 1.65% and 1.09%, respectively. The contents of Chlorogenic acid and Hyperoside were different obviously in samples from different areas. Conclusion This method is simple, rapid and sensitive, and it can be used for the further development and the quality control of the leaves of acanthopanax senticosus.

关 键 词：刺五加叶 绿原酸 金丝桃苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]