生脉注射液对百草枯中毒所致急性肺损伤治疗作用的实验研究  

Empirical study on therapeutical effect of pulse-activating injection to acute lung injury induced by paraquat in rats

作　　者：刘燕琳[1] 刘会芳[2] 赵燕燕[2] 

机构地区：[1]泰山医学院附属医院药剂科,山东泰安271000 [2]河北大学医学部卫生职业技术学院,河北保定071000

出　　处：《泰山医学院学报》2009年第9期656-659,共4页Journal of Taishan Medical College

基　　金：河北省中医药管理局科研基金资助项目(2008070);河北省卫生厅科研基金资助项目(08370);河北大学医学部科学研究基金项目(2007Q06)

摘　　要：目的观察百草枯(PQ)急性中毒大鼠所致肺损伤(ALI)时一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的变化,探讨生脉对急性百草枯中毒所致肺损伤的保护作用。方法将50只SD大鼠随机分成5组,空白组、阴性对照组、阳性对照组、生脉低剂量组和生脉高剂量组。观察大体标本,组织病理以及生物学标志:肺湿/干重比、肺泡灌洗液中性粒细胞比和蛋白含量。同时测定肺组织NO含量和iNOS活性。结果与阴性对照组相比,肺组织病理显示肺淤血、肺水肿明显减轻,其生物学标志降低(P<0.05,P<0.01),NO和iNOS也降低(P<0.05,P<0.01)。结论NO及iNOS在百草枯所致大鼠肺损伤中起重要作用,生脉能降低NO及iNOS水平,减轻百草枯中毒大鼠肺组织损伤。Objective：The variance of NO and iNOS of acute lung injury induced by paraquat on rats was investigated,and the protection of pulse-activating injection in acute lung injury on rat was approached.Methods：50 Sprague-Dawley（SD） rats were randomly divided into five experimental groups： blank group（NS）,negative control group（PQ）,positive control group（D）,pulse-activating injection low-dose group（LDG）,pulse-activating injection high-dose group（HDG）.Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens.The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid,In the mean time,the activities of lung tissue homogenate NO and iNOS were measured.Results： Compared with that in negative control group,lung congestion and lung edema were mitigated.Lung wet weight/dry weight,rat of neutrophils,protein content were decreased（P〈0.05,P〈0.01）;and biological indexes of lung injury and the contents of NO and iNOS were significantly decreased（P〈0.05,P〈0.01）.Conclusion： NO and iNOS could play an important role in lung injury of poisoned rats.Pulse-activating injection could ameliorate the lung injury and decrease the concentrations of NO and iNOS.

关 键 词：生脉注射液 百草枯 肺损伤 一氧化氮 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]