土法造纸木素降解之探索Ⅲ.嫩毛竹自然发酵过程中的细菌分离鉴定与嫩竹软化菌的筛选  

STUDIES ON LIGNIN DEGRADATION BY LOCAL NATIVE METHOD OF PAPER PULP PRODUCTION Ⅲ.Bacterial Isolation and Identification from Natural Fermentation Liquid of Young Bamboo and Selection of Bacteria to Soflen Bamboo

作　　者：傅筱冲[1] 廖延雄[1] 曹晖[1] 吴小琴[1] 刘志连 傅琳琳[1] 任婷娘 

机构地区：[1]江西省科学院

出　　处：《江西科学》1998年第1期12-20,共9页Jiangxi Science

基　　金：江西省自然科学基金

摘　　要：试验证实，嫩毛竹自然发酵过程中的微生物主要是细菌。分离出并纯化了１００株细菌，其中６０株是芽胞杆菌。将分离出的１００株细菌分别接入石灰预处理过的嫩竹，从中筛选出能使嫩竹进一步软化的菌株３株，也均为芽胞杆菌。对这６０株芽胞杆菌进行了形态学及其生理生化特征的鉴定，按Ｐｒｉｅｓｔ氏等（１９８８年）的数值分类系统分类到组，将３株（３０８，３０９，３１０）对嫩竹软化有明显作用的芽胞杆菌的１株（３０８）鉴定到种，认为是短小芽胞杆菌（Ｂ．ｐｕｍｉｌｕｓ）。另外４０株非芽胞杆菌参阅《伯杰氏系统细菌学手册》第１卷和第２卷，鉴定到科或属，有微球菌、葡萄球菌、李氏杆菌、不动杆菌、肠杆菌科等。Experiments proved that the microorganisms in the natural fermentation liquid of young Bamboo for paper pulp making mainly were bacteria. One hundred strains were isolated and identified.There were 60 strains of spore forming rods. The isolated 100 strains inoculated seperatdy into tubes containing cubes of young bamboo which had been treated with lime previously to examine which strain can soften the young bamboo strip. Three strains(308,309,310)were found.They were all Bacillus .The 60 strains of spore forming bacteria were identified by their morphological,physiological and biochemical characteristics.According to Priest et al (1988) 1 numerical taxonomy system,all Bacillus (59 strains)were identified into Priest's group.One strain,308 was classified as B.pumillus .One spore forming bacteria was identified as a sporolactobacillus. The other 40 strains of non spore forming bacteria were identified as Micrococcus,Staphylococcus,Listeria,Acinatobacter,Enterobacteriaceae etc.according to Bergey's Systematic Bacteriology Vol.I and Vol.Ⅱ 8,10 .

关 键 词：嫩竹 土法造纸 造纸 毛竹 自然发酵 木素 降解 

分 类 号：TS743.9[轻工技术与工程—制浆造纸工程] TS721.2