藏红花愈伤组织诱导和褐化抑制  

Callus induction and browning inhibition of Crocus sativus L.

作　　者：袁丽红[1] 陆玉婷[1] 黄晶[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2009年第6期21-26,共6页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20576055)

摘　　要：对藏红花的球茎、无菌芽和幼叶的愈伤组织诱导条件进行研究,同时探讨愈伤组织诱导过程中的褐化和污染问题.结果表明:与球茎相比,以无菌芽和幼叶为外植体,愈伤组织诱导率较高,诱导时间较短,但形成的愈伤组织易褐化.较大球茎(≥20 g)也是藏红花愈伤组织诱导较好的外植体,愈伤组织诱导速度随球茎储藏时间的增加而升高.含有2.0 mg/L 2,4-二氯苯氧乙酸(2,4-D)和0.5 mg/L 6-苄氨基嘌呤(6-BA)的MS(Murash ige and Skoog)培养基有利于球茎愈伤组织的诱导,30 d愈伤组织诱导率可达70%以上.在愈伤组织诱导过程中,连续转接外植体至新鲜培养基可以有效降低褐化率,而在培养基中添加活性炭(AC)和聚乙烯吡咯烷酮(PVP)不能有效抑制褐化.表面灭菌前采用流水冲洗过夜可有效减少球茎表面附生菌,明显降低污染率.Callus induction from corms, sterile buds and young leaves of Crocus sativus L. was studied, and the browning and contamination in the callus induction were also explored. Results indicated that with the sterile buds and young leaves as explants, the percentage of callus inducement was higher and the inducing time was shorter than that with corms as explants, but the browning took place easily in callus. The bigger corms （≥20 g） were suitable for the callus induction. Moreover, the longer corms were stored, and the faster calli were induced. The percentage of callus inducement was over 70% within 30 d by using Murashige and Skoog （MS） medium supplemented with 2.0 mg/L 2,4-D and 0. 5 mg/L 6-BA. Inoculating explants to fresh media in succession could effectively reduce the browning, whereas media with the addition of activated carbon and polyvinylpyrrolidone were ineffective to diminish the browning. Washing corms with running tap water overnight before sterilization were effective for reducing surface epiphytic microorganisms significantly and depressing the rate of contamination.

关 键 词：藏红花 球茎 愈伤组织 诱导 褐化 

分 类 号：O611.62[理学—无机化学]