乙醇与甲醇对中华大蟾蜍坐骨神经动作电位影响的比较研究  

A Comparative Study on the Effects of Ethanol and Methanol on Action Potential of Toad′ Sciatic Nerve

作　　者：李小芳[1] 杨志松[2] 黄小富[2] 董丽[2] 

机构地区：[1]西昌学院生化系,四川西昌615022 [2]西华师范大学生命科学学院,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2009年第4期392-397,共6页Journal of China West Normal University(Natural Sciences)

基　　金：四川省重点学科建设项目(SZD0420);西华师范大学校内科研项目(06A008)

摘　　要：应用电生理方法观察了不同浓度的乙醇和甲醇对中华大蟾蜍离体坐骨神经干刺激阈值、最大刺激值、动作电位峰值和传导速度等四种生理指标的影响.结果表明:低浓度的乙醇(0.1%,0.3%,0.5%,1%,3%,5%)对神经干刺激阈值和最大刺激值的影响不明显,能引起动作电位峰值和传导速度先缓慢上升再缓慢下降;中、高浓度的乙醇(8%,10%,12%)对上述四种生理指标影响显著,随着浓度的递增,刺激阈值和最大刺激值逐渐增大,动作电位峰值逐渐下降,传导速度逐渐变慢.甲醇随着浓度的递增,对神经干上述四种生理指标的影响与乙醇基本一致,只是同浓度的甲醇较乙醇影响更为显著.这说明,甲醇对坐骨神经干动作电位的抑制作用要大于乙醇.With electrophysiological methods, the effects stimulus threshold, maximal stimulus, peak value and of different concentrations of ethanol and methanol on the conduction velocity of action potential of isolated sciatic nerve in toad were observed. The results showed that：low concentrations of ethanol （0.1% , 0.3% , 0.5% , 1% , 3% , 5 % ） hadnot obvious effects on the stimulus threshold and maximal stimulus （ compared with the control）, and caused peak value and conduction velocity of action potential increasing slowly, and then decreasing slowly successively. Whereas the high concentration of ethanol （8% , 10% , 12% ） had significant impact on the above four physiological indicators. With the increasing concentration, the stimulus threshold and maximal stimulus in- creased gradually, but peak value of action potential decreased gradually and conduction velocity slowed down gradually. With the increasing concentration, the influence of methanol on the above four physiological indicators was the same as that of ethanol basically, but the physiological effects of methanol was more obvious than the same concentration of ethanol. It indicated that the inhibitory effect of methanol on sciatic nerve was greater than that of ethanol.

关 键 词：乙醇 甲醇 中华大蟾蜍 坐骨神经动作电位 

分 类 号：Q463[生物学—生理学] Q959.53