高效液相色谱波长转换法测定西红花苷-1和栀子苷的含量  

Determination of Dafflower Glucoside-1 and Jasminoidin in Gardenia by RE-HPLC

作　　者：付建武[1] 彭红[1] 周玉春[1] 黄丽芸[1] 

机构地区：[1]江西中医学院,江西南昌330004

出　　处：《时珍国医国药》2009年第12期2939-2940,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技部支撑计划课题(No.2006BAI06A07)

摘　　要：目的采用HPLC波长转换法测定栀子药材中西红花苷-1和栀子苷的含量。方法色谱柱为Agilent Zorbax E-clipse XBD-C18,流动相为乙腈∶水(梯度洗脱),流速为1 ml/min,检测波长为238 nm(0～20 min);440 nm(20～40min),柱温为30℃,采用20 min转换检测波长。结果栀子苷在4.52～180.8μg范围内,r=0.999 9,西红花苷-1在0.9～9.0μg范围内,r=0.999 7,与色谱峰面积呈线性关系。平均回收率分别为99.52%,100.76%,RSD分别为1.10%,1.41%。结论该测定方法具有简便、稳定、准确、重复性好的特点。Objective To determine the jasminoidin and safflower glucoside - 1 of Gardenia. Methods Using RE - HPLC with Agient Zorbax Eclipse XBD- C18 column,the mobile phase was composed of a mixture of acetonitrilewater（grads elution）. The flow rate was 1 ml/min, and the UV detection wavelengths were set at 238nm （ 0 - 20 rain） and 440nm （20 - 40 min） , and the tempetature was at 30℃. The wavelength was converted at 20min. Results Irisflorentins of the drugs showed linear relationship with peak areas in the range of 4.52 - 180 μg/ml and 0.9 - 9.0μg/ml respectively（ r = 0. 999 9,0. 999 7 ）. The average recoveries were 99.52% and 100.76%. The RSD were 1.10% and 1.41%. Conclusion The method is simple,stable,accurate and repeatable.

关 键 词：高效液相法 栀子苷 西红花苷-1 

分 类 号：R284.1[医药卫生—中药学]