达肝清对小鼠肝细胞异常凋亡的影响  

Effects of Chinese Herb Compound Daganqing on Apoptosis of Liver Cells in Mice

作　　者：郝二伟[1] 邓家刚[2] 阎莉[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]广西中医学院,广西南宁530001

出　　处：《时珍国医国药》2009年第12期3049-3050,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西研究生教育创新计划项目(No.2006106001008M01)

摘　　要：目的探讨中药复方达肝清对苯巴比妥钠诱导的小鼠肝细胞异常凋亡的影响,进一步揭示其抗肝损伤的作用机理。方法采用撤除苯巴比妥钠(PB)诱导小鼠肝细胞凋亡的模型;通过流式细胞检测技术,TUNEL原位检测法,HE常规染色等方法,以肝细胞凋亡率、肝组织形态、原位细胞TUNEL反应、肝/体重比为指标,观察不同剂量达肝清对PB诱导肝细胞凋亡的影响。结果流式细胞仪分析表明,达肝清高、中剂量组肝细胞凋亡率明显低于凋亡对照组;达肝清高剂量组肝/体重比回落明显低于凋亡对照组;组织学染色及TUNEL反应,凋亡对照组可见凋亡小体,凋亡现象明显,凋亡细胞明显多于达肝清高、中剂量组。结论达肝清能明显抑制苯巴比妥钠诱导的肝细胞凋亡,进一步提示抑制肝细胞异常凋亡可能为其抗肝损伤的机理之一。Objective To study the effects of Chinese herb compound Daganqing on apoptosis caused by phenobarbital withdrawal of liver cells in mice, and to research the mechanism of its preventing acute liver injury. Methods The apoptosis model of liver cells in mice caused by phenobarbital withdrawal was used, FCM, TUNEL, and HE routine dye, were performed to check the changes in apoptotic cells. The rate of hepatocyte apoptosis, the hepatic histopathologic changes, tunnel change and liver body weight ratio were observed to study the effects of Daganqing in different dosages on apoptosis caused by phenobarbital withdrawal of liver cells. Results Flow cytometry analysis revealed that the apoptotic rate of liver cells and liver body weight ratio of Daganqing high and middle dose groups were obviously lower than positive group. By the analysis of TUNEL, and HE routine dye, apoptotic bodies and cell apoptosis were more conspicuous, and the number of apoptotic cell was more in positive group than other gYoups. Conclusion Chinese herb compound Daganqing in the dosages of 34 g/kg, 17 g/kg can obviously inhibit the liver eell apoptosis caused by phenobarbital withdrawal, and further reveal that inhibiting the liver cell apoptosis maybe one of the mechanisms of Daganqing preventing acute liver injury.

关 键 词：达肝清 肝细胞凋亡 流式细胞术 原位末端缺口标记 

分 类 号：R285.5[医药卫生—中药学]