升压宁对内毒素休克肝损伤大鼠肿瘤坏死因子-α和一氧化氮合酶的影响  

The Effect of Sheng Ya Ling on TNF-α and iNOS in Liver Injury Rats with Endotoxic Shock

作　　者：郑秀丽[1] 冯全生[1] 杨宇[1] 张琦[1] 乔胃娟[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《时珍国医国药》2009年第12期3069-3071,共3页Lishizhen Medicine and Materia Medica Research

基　　金：四川省中医药管理局课题(No.2004A08)

摘　　要：目的观察升压宁对内毒素休克肝损伤大鼠肝组织中肿瘤坏死因子-α(TNF-α)和诱导型一氧化氮合酶(iNOS)的影响。方法采用一次性静脉注射内毒素(LPS)10 mg/kg复制内毒素休克模型,以升压宁预处理动物,并以地塞米松为阳性对照,免疫组化法测定肝组织中TNF-α和iNOS的表达。结果内毒素休克模型组存在严重肝损伤,肝组织中TNF-α和iNOS表达较空白对照组显著增强(P(0.01),升压宁组肝损伤较模型组减轻,TNF-α和iNOS的表达与模型组比较显著降低(P(0.01)。结论升压宁能减轻内毒素休克肝损伤,其机理与抑制TNF-α和iNOS的表达有关。Objective To explore the effect of Sheng Ya Ling on TNF - α and iNOS in liver injury rats with endotoxic shock. Methods The rats were injected intravenously LPS 10mg/kg to make endotoxie shock models. They were pre -treated by Sheng Ya Ling with the rats given dexamethasone as positive control group. Then observed the expression of TNF - α and iNOS were measured by immunohistochemical method. Results In the endotoxie shock model group, livers of rats were severely injured and the expression of TNF-α and iNOS in livers were increased more significantly than that in the blank control group（ P 〈 0.01 ）. The liver injury of Sheng Ya Ling group was slighter than that of model group and expression of TNF - α and iNOS were decreased more significantly than that of model group（ P 〈 0.01 ）. Conclusion Sheng Ya Ling can reduce liver injury, by endotoxic and its mechanism may be with inhabitation of expression of TNF - αand iNOS.

关 键 词：升压宁 内毒素休克 肝损伤 生脉散 

分 类 号：R285.5[医药卫生—中药学]