高效液相色谱法测定地黄寡糖中水苏糖含量  

Determination of Stachyose Contents in Rehmannia Glutinosa Oligosaccharides by High Performance Liquid Chromatography

作　　者：安阳[1] 杨菁[1] 岳春丽[1] 刘影[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《时珍国医国药》2009年第12期3071-3072,共2页Lishizhen Medicine and Materia Medica Research

基　　金：辽宁省自然科学基金(No.20072202);辽宁省教育厅资助课题(No.20060528)

摘　　要：目的建立高效液相色谱法测定地黄寡糖中水苏糖含量方法。方法采用菲罗门Rezex RPM-Monosaccharide Pb++8%(7.8mm×300mm)色谱柱,以水为流动相,流速0.8 ml/min,示差检测器测定地黄寡糖中水苏糖含量。结果在该色谱条件下,水苏糖在0.20～3.20 mg/ml范围内峰面积和进样质量有良好的线性关系,其相关系数为0.999 5。样品平均回收率为99.9%,RSD为1.2%(n=6)。结论该方法灵敏可靠,专属性强,且具有快速、简便、重复性好的特点。Objective To establish an HPLC method for determination of stachyose contents in Rehmannia Glutinosa Oligosaccharides（ROS）. Methods Stachyose was determined with Rezex RPM -Monosaccharide Pb ＋ ＋ 8% （7.8 mm× 300 mm）column, water as mobile phase and differential refractive index detector. Results The correlation coefficient between the area of stachyose and stachyose content was 0. 999 5. The sample average recovery was 99.9% , the RSD was 1.2 % （ n = 6）. Conclusion This method is simple, rapid, specific, accurate and reproducible.

关 键 词：地黄寡糖 水苏糖 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]