宁强矮马生长激素基因(GH)的遗传变异分析  

Genetic Variation of Growth Hormone Gene on Ningqiang Pony

作　　者：张涛[1] 路宏朝[1] 陈锐[1] 周建军[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723001

出　　处：《中国农业科学》2009年第12期4435-4440,共6页Scientia Agricultura Sinica

基　　金：陕西省教育厅科研项目(07JK212);陕西理工学院科研项目(SLGQD0513)

摘　　要：【目的】通过分析不同动物和中国几个地方马种生长激素基因序列,为宁强矮马起源和矮化形成机制的研究提供分子水平的试验依据。【方法】通过PCR扩增宁强矮马生长激素基因并进行测序,用DNAMAN软件对不同动物的生长激素基因序列进行比对分析。【结果】马品种间29个位点发生遗传变异,编码区11个位点发现遗传多态现象。以蒙古马生长激素氨基酸序列为标准,宁强矮马仅在1765位点的变异导致氨基酸发生改变。以生长激素的mRNA序列为基本数据进行聚类分析,宁强矮马和德保马67的同源性为100%,与百色马的同源性为99.8%,与蒙古马的同源性为99.7%。【结论】马属动物生长基因序列与猪和狗的同源性较高,宁强矮马1765位的G→A可能影响到宁强矮马的生长发育。【Objective】 In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony,the sequence of GH of different animals and some China horse breeds were studied.【Method】 The PCR amplification,sequencing and DNAMAN software were used.【Result】 There were 29 genetic variation,11 loci were found in the coding region.Taking the AA sequence of GH of Mongolia horse as standard,there was 1 amino acid variation in Ningqiang pony.Through clustering analysis based on the sequence of mRNA of GH,the homology was 100%,99.8%,99.7%,between Ningqiang and Debao 67,Baise and Mongolia.【Conclusion】 The homology level of GH was higher among Equus caballus,Sus scrofa and dog in present study,and the mutation of G→A affects the growth of Ningqiang pony.

关 键 词：宁强矮马 生长激素基因 序列分析 遗传变异 

分 类 号：S821[农业科学—畜牧学]