银杏叶提取物对大鼠颅脑损伤后心肌病变的保护作用及神经肽Y基因表达的影响  

Protective effects of GBE on myocardial damage after traumatic brain injury in rats and on the expression of NPY mRNA in myocardium

作　　者：郝春艳[1] 李茹香[1] 段虎斌[2] 

机构地区：[1]山西医科大学第一医院老年病科,太原030001 [2]山西医科大学第一医院重症医学科,太原030001

出　　处：《中国药物与临床》2009年第12期1156-1158,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金(30600637)

摘　　要：目的观察银杏叶提取物(GBE)对大鼠颅脑损伤后心肌病变的保护作用及神经肽Y(NPY)基因表达的影响。方法采用液压冲击建立颅脑损伤模型和药物干预模型,行心导管插管测定左心室收缩压(LVSP),左心室舒张压末压(LVEDP)及左心室压力上升和下降最大速度(±dp/dtmax),同时测定血清肌酸磷酸激酶同工酶(CK-MB)含量的改变,并通过反转录-聚合酶链反应(RT-PCR)的方法研究大鼠颅脑损伤后心肌NPY基因的表达。结果与对照组大鼠比较,颅脑损伤组大鼠LVSP和±dp/dtmax明显下降(P<0.05),LVEDP明显升高(P<0.05),血清CK-MB含量明显升高(P<0.05),且心肌NPY基因的表达水平显著增加(P<0.05);与颅脑损伤组大鼠比较,GBE干预组大鼠LVSP和±dp/dtmax增加(P<0.05),LVEDP降低(P<0.05),血清CK-MB含量降低(P<0.05),且心肌NPY基因的表达水平减少(P<0.05)。心肌NPY基因的表达与血清CK-MB和LVEDP变化呈正相关(r值分别为0.988和0.957,P<0.05),与LVSP变化呈负相关(r=-0.960,P<0.05)。结论颅脑损伤可引起明显的心肌病变,GBE对大鼠颅脑损伤后心肌病变有保护作用,其作用可能与心肌NPY基因表达下调有关。Objective To explore the protective effects of Ginkgo Biloba extract （GBE） on myocardial damage after traumatic brain injury in rats and on the expression of neuropeptide Y （NPY） mRNA in myocardium. Methods Fluid percussion induced TBI models and GBE-pretreated models were established in rats. Left ventrieular systolic pressure （LVSP）, left ventricular end diastolic pressure （LVEDP） and ＋ dp/dtmax were measured by multipurpose polygraph under left cardiac catheterization. The level of MB isoenzyme of creatine kinase （CK-MB） in serum was also determined. The expression of NPY in myocardium was detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with control group, LVSP. and ＋dp/dtmax were lowered （P〈0.05）, whereas LVEDP, level of CK-MB and expression of NPY in myoeardium were significantly higher in the TBI group （all P〈 0.05）. Compared with TBI group, GBE-pretreated group showed higher levels of LVSP and ＋dp/dtmax （both P〈0.05）, lowered LVEDP, level of CK-MB and expression of NPY in myocardium （all P〈0.05）. Correlation analysis showed that the expression of NPY in myocarclium was positively correlated with the level of CK-MB （r=0.988,P〈0.05） and LVEDP （r=-0.957, P〈0.05）, and the expression of NPY in myocardium was negatively correlated with LVSP （r=-0.960, P〈0.05）. Conclusi6n Traumatic brain injury can result in myocardial damage, and GBE can protect myocardium by down-regulating the expression of NPY in myocardium.

关 键 词：颅脑损伤 心肌疾病 神经肽Y 

分 类 号：R285.5[医药卫生—中药学]