人脂肪干细胞向脂肪细胞的定向分化  

Differentiation of human adipose-derived stem cells into adipocytes

作　　者：杨旭芳[1,2] 何旭[1] 何牮[1] 张丽红[1] 李玉林[1] 

机构地区：[1]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021 [2]牡丹江医学院病理生理学教研室,黑龙江牡丹江157011

出　　处：《吉林大学学报（医学版）》2009年第6期987-991,F0002,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30700872);国家863重大专项基金资助课题(2004AA205020);吉林大学青年教师基金资助课题(419070100050)

摘　　要：目的:分离和培养人脂肪干细胞(hADSCs),并在体外定向诱导hADSCs分化为脂肪细胞。方法:利用胶原酶消化法和贴壁筛选法从人脂肪组织中分离、培养及扩增hADSCs;应用地塞米松、吲哚美辛、3-异丁基-1-甲基黄嘌呤及胰岛素定向诱导hADSCs分化为脂肪细胞。结果:体外分离、培养出高度同源性的hADSCs,hADSCs具有相对特异的细胞表面标志,即CD29、CD73、CD105及CD166呈阳性表达,而CD31及HLA-DR呈阴性表达;hADSCs具有典型的干细胞增殖特点,大部分处于静息期,只有少数细胞处于增殖活跃期;hADSCs能被诱导分化为脂肪细胞。结论:成功地分离、培养出高度同源性的、未分化的hADSCs;体外定向诱导hADSCs可分化为脂肪细胞。Objective To find the most effective way to obtain highly homogenous and undifferentiated human adipose-derived mesenchymal stem cells（hADSCs）,and induce the hADSCs to differentiate into adipocytes.Methods hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.hADSCs were differentiated into adipocytes by using dexamethasone,indomethacin,IBMX and insulin.Results hADSCs had unique immunophenotypes and they were positive for CD73,CD44,CD166,CD105 and CD29,but negative for CD31,CD34,CD45 and HLA-DR.hADSCs had the typical proliferative characteristics of stem cells,most of them were in the resting phase ,only a few cells were in the active proliferative phase.They could be induced to differentiate into adipocytes.Conclusion It is successful to isolate and obtain highly homologous hADSCs,and it was also successful in inducing the hADSCs to differentiate into adipocytes in vitro.

关 键 词：干细胞 流式细胞术 脂肪细胞 

分 类 号：Q813.1[生物学—生物工程]