Bcl-2及Bax在异丙肾上腺素致心肌坏死模型大鼠心肌中的表达及意义  

Expressions of Bcl-2 and Bax in myocardium of rats with isoproterenol-induced myocardial necrosis and significances

作　　者：李相军[1] 杨莉[1,2] 任立群[1] 石艳[1] 孙波[1] 苗春生[1] 

机构地区：[1]吉林大学药学院实验药理与毒理学教研室,吉林长春130021 [2]内蒙古通辽市医院功能检查科,内蒙古通辽028000

出　　处：《吉林大学学报（医学版）》2009年第6期1044-1047,I0012,共5页Journal of Jilin University：Medicine Edition

基　　金：教育部博士点基金资助课题(200801830050)

摘　　要：目的:探讨Bcl-2及Bax在异丙肾上腺素(Isp)致心肌坏死模型大鼠心肌中的表达及意义。方法:40只大鼠随机分为4组:正常对照组及注射Isp后12h、1周和3周组。除正常对照组外,其他各组大鼠皮下注射Isp(15mg·kg-1体重),并于给药后12h、1周和3周时取对应组大鼠心脏,用免疫组化法及RT-PCR法检测各组大鼠心肌组织中Bcl-2及Bax的表达。结果:免疫组化结果显示,注射Isp后12h及1周组大鼠心肌组织中Bax表达明显高于正常对照组(P<0.05);注射Isp后12h、1周及3周组大鼠心肌组织Bcl-2表达量均明显低于正常对照组(P<0.05);注射Isp后12h、1周、3周后,各组大鼠心肌组织Bcl-2/Bax比值均低于正常对照组(P<0.05)。RT-PCR结果显示,注射Isp后各组大鼠心肌组织Bax mRNA表达量均明显高于正常对照组(P<0.05),且在注射Isp后1周时,Bax mRNA表达量达到高峰;注射Isp后,各组大鼠心肌组织Bcl-2mRNA表达量均明显低于正常对照组大鼠(P<0.05);与正常对照组大鼠比较,在注射Isp后12h及1周,各组大鼠心肌组织Bcl-2/Bax比值明显增高(P<0.05)。结论:心肌细胞的凋亡是心肌纤维化的病理基础,而Bcl-2家族基因及蛋白表达变化在此过程中起主要调控作用。Objective To detect the expressions of Bcl-2 and Bax gene and protein in myocardium of rats with isoproterenol （Isp）-induced myocardial necrosis and significances.Methods 40 Wistar rats were divided into control group,12 h,1 week and 3 weeks after Isp injection groups.Except control group,all the rats in the other groups were treated with isoproterenol hydrochloride.12 h,1 week and 3 weeks after Isp injection,immunohistochemistry and RT-PCR were applied to detect the gene and protein expressions of Bcl-2 and Bax.Results The results of immunohistochemistry showed that 12 h and 1 week after Isp injection,the expressions of Bax were increased significantly compared with control group （P〈0.05）;Inversely,the expressions of Bcl-2 were decreased significantly 12 h,1 week and 3 weeks after Isp injection compared with normal control group （P〈0.05）.The ratios of Bcl-2/Bax were significantly decreased 12 h,1 week and 3 weeks after Isp injectioncompared with control group （P〈0.05）. The expressions of Bax mRNA were increased significantly 12 h,1 week and 3 weeks after Isp injection compared with control group （P〈0.05）;The expressions of Bcl-2 were decreased significantly 12 h and 1 week after Isp injection compared with control group （P〈0.05）.Conclusion Apoptosis of myocardium is the basic pathological change of myocardial fibrosis,and the expressions of gene and protein of Bcl-2 family play an important role in this process.

关 键 词：心肌坏死 细胞调亡 基因 BCL-2 BAX 

分 类 号：R542.22[医药卫生—心血管疾病]