微量元素作为甲醛损伤生物标志物的水平分析  

Analysis of levels of microelements as biomarker in injury induced by formaldehyde

作　　者：于敬红[1] 李环[1] 孙维琦[1] 徐晓磊[1] 高久春[2] 

机构地区：[1]北华大学公共卫生学院环境卫生教研室,吉林吉林132001 [2]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第6期1065-1067,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200505248)

摘　　要：目的:通过观测甲醛对小鼠肝脏、全血微量元素含量的影响,探讨甲醛损伤的生物标志物。方法:80只健康昆明小鼠随机分为对照组及甲醛染毒低剂量组(1/40LD50)、中剂量组(1/20LD50)和高剂量组(1/10LD50),每组20只,采用吸入染毒方式,每天2h,连续染毒3及6周后断头处死,分别测定小鼠肝脏和全血Cu、Fe、Zn、Mn的含量。结果:染毒3周高剂量组Fe、Zn、Mn含量及染毒6周中、高剂量组小鼠肝脏Fe、Zn含量明显低于对照组(P<0.05),染毒3周高剂量组小鼠全血Mn、Zn含量明显低于对照组(P<0.05),染毒6周后中、高剂量组小鼠全血Fe、Zn明显低于对照组(P<0.05);染毒不同时间的各剂量组小鼠肝脏及全血Cu含量均无显著变化(P>0.05)。结论:甲醛可引起小鼠肝脏及全血Fe、Zn及Mn含量降低,Mn、Zn及Fe可作为甲醛损伤的标志物。Objective To study the effects of formaldehyde on the level of microelements in mouse liver and blood and explore the microelements as biomarker in injury induced by formaldehyde.Methods The mice were randomly divided into control,low（1/40LD50）,moderate（1/20LD50） and high（1/10LD50） dose groups exposed to formaldehyde by inhalation（n=20）,two hours per day for three and six weeks,and than the contents of Cu,Fe,Zn and Mn in mouse liver and blood were measured.Results The contents of Fe,Zn and Mn in liver in high dose group three weeks after exposure and the contents of Fe and Zn in liver in high dose group six weeks after exposure were significantly lower than those in control group（P〈0.05）;the contents of Mn and Zn in blood in high dose group three weeks after exposure were significantly lower than those in control group（P〈0.05）;while six weeks after exposure,the contents of Fe and Zn in blood in moderate and high dose groups were significantly lower than those in control group（P〈0.05）;and the content of Cu had no significant change in each group.Conclusion Formaldehyde might decrease the contents of Fe,Zn and Mn in mouse liver and blood,and the Fe,Zn and Mn could be used as biomarkers in injury induced by formaldehyde.

关 键 词：甲醛 微量元素 生物标志物 

分 类 号：R135.14[医药卫生—劳动卫生]