痛必定冻干粉针对脊髓中枢的镇痛作用及其机制  

Analgesia effect of Tongbiding free-dried powder for injection on spinal cord center and its mechanism

作　　者：曹军[1] 王中男[2] 孙淑娟 

机构地区：[1]吉林公安高等专科学校刑事技术教研室,吉林长春130117 [2]长春中医药大学,吉林长春130117 [3]一汽集团总医院心内科,吉林长春130011

出　　处：《吉林大学学报（医学版）》2009年第6期1088-1091,共4页Journal of Jilin University：Medicine Edition

基　　金：国家教育部重点研究项目资助课题(205036)

摘　　要：目的:研究痛必定冻干粉针对慢性结扎损伤(CCI)模型活体大鼠脊髓在体WDR神经元的作用,阐明痛必定的脊髓中枢镇痛机理,从细胞水平研究痛必定是否具有药物依赖性。方法:采用电生理细胞外记录技术,在CCI活体大鼠的脊髓腰膨大节段,寻找并记录给予痛必定20mg·kg-1前以及给药后不同时间段同一活体WDR神经元的电活动:观察给药前以及给药后2、4、8和10min同一活体WDR神经元的C反应放电数;观察给药前及给药后1、2、4和6min同一活体细胞的自发放电数;观察wind-up现象。结果:C反应放电数共记录到8个细胞,给药后2、4、8和10min与给药前平均C反应放电数比较差异有显著性(P<0.01或P<0.05)。观察细胞的自发放电数共记录到12个细胞,其中6个在给药后立即停止自发放电,6个在给药后6min以内放电停止,给药后2和4min与给药前平均自发放电数比较差异有显著性(P<0.01)。实验共观察到5次wind-up现象,给药后此现象均被抑制。结论:痛必定冻干粉针的脊髓中枢镇痛是通过对WDR神经元的作用来完成的,本研究从细胞水平证实痛必定无药物依赖性。Objective To study the effect of Tongbiding freezing-dryied powder for injection on the function of WDR neuron of spinal cord in living rats with chronic constriction injury （CCI）,and clarify its analgesia mechanism in spinal cord center,and further explore whether it has the drug dependence at the cellular level. Methods Electricity physiology extracellular recording technique was used ,in the CCI living rat＇s expanded spinal cord waist stage the electricity activity of identical WDR neuron cells was recorded before and after administration of 20 mg·kg-1 Tongbiding. The electric discharge number of C response was observed before and 2,4,8,10 min after administration. The spontaneous electric discharge number. was observed before and 1,2,4,6 min after administration; wind-up phenomenon was also observed.Results The electric discharge number of C response was obtained in 8 WDR neurons,three were significant differences of the mean electric discharge number of C response between 2,4,8 min after administration and before administration （P〈0.05 or P〈0.01）. The observation of cell＇s spontaneous electric discharge number recorded 12 cells altogether,6 cells stopped discharging spontaneously immediately after administration,6 cells stopped discharging spontaneously 6 min after administration. There were significant differences of the mean spontaneous electric discharge number between 2,4 min after administration and before administration （P〈0.01）.Five wind-up phenomena were found altogether,this phenomenon was suppressed after. Conclusion The analgesia effect of Tongbiding freezing-dryied powder for injection on spinal cord center is related to affecting the WDR neuron ,and it is confirmed that the drug does not have drug dependence at the cellular level.

关 键 词：痛必定冻干粉针 CCI模型大鼠 WDR神经元 电活动 

分 类 号：R285.5[医药卫生—中药学]