云南白药对大鼠骨骼肌急性损伤后炎症反应和卫星细胞再生的影响  

Effects of Yunnan Baiyao on inflammatory response and satellite cell regeneration after acute injury of skeletal muscle in rats

作　　者：孙茹 赵立君[2] 杨熹洁[3] 邴国强[1] 

机构地区：[1]吉林大学体育学院运动人体科学教研室,吉林长春130012 [2]吉林大学体育学院术科教研室,吉林长春130012 [3]长春师范学院体育学院人体生物学教研室,吉林长春130032

出　　处：《吉林大学学报（医学版）》2009年第6期1092-1096,I0012,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20000200)

摘　　要：目的:研究云南白药对大鼠急性骨骼肌损伤后炎性细胞和肌再生细胞(卫星细胞)的作用及其机理。方法:制备跑台诱导大鼠骨骼肌急性损伤模型,造模24h后,模型大鼠左侧后肢喷洒云南白药为治疗侧,右侧喷洒等量生理盐水作为非治疗侧,在损伤后2、4、7、10和14d分别取4只大鼠后肢的治疗侧和非治疗侧腓肠肌(快肌)和比目鱼肌(慢肌)做HE切片,在光学显微镜下观察炎性细胞和卫星细胞的数量及形态学变化。结果:药物治疗侧快肌在损伤后的第2、4、7天炎性细胞较非治疗侧明显减少(P<0.01),肌细胞的坏死程度较轻;云南白药治疗侧慢肌在损伤后的第2天炎性细胞较非治疗侧明显减少(P<0.01)。药物治疗侧的快肌和慢肌在损伤后的第7、10天损伤的肌纤维部分得到修复,卫星细胞的数量较非治疗侧明显增多(P<0.01)。结论:云南白药能够抑制炎性细胞对损伤后肌肉组织的破坏作用,缩短炎症反应进程,促进损伤后肌纤维的修复,对骨骼肌卫星细胞的增殖有一定的促进作用。Objective To study the effects and mechanism of Yunnan Baiyao on the inflammatory response and satellite cell regeneration after acute injury of skeletal muscle in rats. Methods Rat models of skeletal muscle injury （gastrocnemius,fast twich;soleus,slow twich） induced by treadmill were constructed 24 h after establishment of the models,the left sides of hind limbs in rats were treated with Yunnan Baiyao,and the right sides were treated with saline at the equal volume.4 rats were chosen 2,4,7,10,14 d after injury,respectively,and gastrocnemius and soleus of the treated sides （the left sides） and untreated sides （the right sides） from hind limbs of 4 rats were obtained,and stained with immunohistochemistry and HE. The quantity and morphological changes of inflammatory cells and satellite cells were observed under optical microscope. Results Compared with untreated sides,the number of inflammatory cells of fast twich after injury in the treated sides was decreased obviously at the 2nd,4th,7th day after injury（P〈0.01） and the number of inflammatory cells of slow twich at the 2nd day after injury in treated sides was decreased （P〈0.01）;the necrotic cells of skeletal muscle were decreased.Compared with untreated sides the muscle fibers of fast twich and slow twich were repaired at the 7th,10th day,and the number of satellits cells was increased obviously in the treated sides（P〈0.01）. Conclusion Yunnan Baiyao can inhibit the destruction of inflammatory cells to injuried muscle tissues,shorten the inflammatory reaction process and promote the proliferation of satellite cells and muscle fiber repairment after injury.

关 键 词：云南白药 急性骨骼肌损伤 炎性细胞 卫星细胞 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]