宫颈癌HeLa细胞中Bcrp1^+、CK17^+及CD44v5^+表型细胞的检测与分离  

Isolation and detection of CK17^+,CD44v5^+ and Bcrp1^+ cells in HeLa cell line of cervical carinoma

作　　者：张松灵[1] 张晓霞[1] 于晓伟[1] 李玉林[2] 

机构地区：[1]吉林大学第一医院妇产科,吉林长春130021 [2]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第6期1127-1130,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：教育部科学技术研究重点项目资助课题(01058);吉林省科技厅发展计划项目资助课题(白求恩基金200705155)

摘　　要：目的:检测Bcrp1、CK17及CD44v5在宫颈癌HeLa细胞中的表达情况及相互关系,推测该3种抗体成为宫颈癌干细胞标记物的可能。方法:采用免疫荧光标记物流式细胞仪分选并检测HeLa细胞中的CK17+、CD44v5+、Bcrp1+表型细胞;利用免疫细胞化学染色法,检测Bcrp1+及Bcrp1-1两组HeLa细胞中CK17及CD44v5的表达情况。结果:HeLa细胞中存在CD44v5+细胞,所占比例为0.1%,CK17+细胞为0.7%,Bcrp1+细胞为11%;Bcrp1+组HeLa细胞中存在CK17+及CD44v5+细胞,而Bcrp1-组中则无,组间比较差异有显著性(P<0.05)。结论:表达CK17及CD44v5的HeLa细胞同时表达Bcrp1转运蛋白,CK17及CD44v5均有可能成为宫颈癌干细胞的标记物。Objective To identify and isolate the CK17^＋,CD44v5^＋ and Bcrp1^＋ cells in HeLa cells,and investigate the relationship between them and select cervical cancer stem cell markers.Methods Flow cytometry was used to identify and isolate the CK17^＋,CD44v5^＋ and Bcrp1^＋ cells in HeLa cells;after isolation,the expressions of CK17 and CD44v5 in Bcrp1^＋ and Bcrp1-phenotype HeLa cells were measured by immunocytochemistry.Results HeLa cells contained 11% Bcrp1＋ cells,0.7% CK17＋ cells and 0.1%CD44v5^＋ cells.Bcrp1^＋ HeLa cells contained CK17^＋ and CD44v5^＋ HeLa cells,but the Bcrp1-Hela cells did not（P〈0.05）.Conclusion HeLa cells expressed CK17 and CD44v5 antibody must express Bcrp1 antibody.CK17 and CD44v5 may be used as the markers of cervical cancer stem cells.

关 键 词：HELA细胞 癌 鳞状细胞 肿瘤干细胞 CK17 CD44V5 Bcrp1 流式分选 

分 类 号：R737.33[医药卫生—肿瘤]